Piping is a consequence of differential head and differential pore pressure

Ideally, the internal levee should maintain a factor of safety of above 1.4, and as such, the maximum back flooding of the system will be will be MSL + 6 ft. Table 3 shows that for different river water levels increasing the back flood water levels results in a increase in the the factor of safety of the levees. While there are minor variations to this trend, the levee becomes more stable with increased back flooding. This result supports the theory that the hydraulic exit gradient resulting in piping and slope instability can be reduced by the Water Management System. The optimum back flooding levels from simulations of various elevated river conditions are highlighted in Table 3. Significantly higher factors of safety for the existing sections of the Sherman Island levees can be achieved with these levels of back flooding resulting in greater levee stability and lower risk of failure from river flooding. The driving forces for levee and piping resulting in slope failure stems from high exit gradients.As pressure builds up on one side of the levee, stacking flower pot tower water is forced towards the backside of the levee and thus increases the hydraulic gradient. This increases the already high hydraulic gradient of the peat layer even further.

The addition of the temporary flood storage zone allow for siphoning water to balance water levees on each side on each side of the level. Therefore the exit gradient and pore pressures are decreased leading to a significant increase of the stability of the levees. Figure 7 compares the exit gradient for an 8+MSL flood level and back flooding of 2+MSL which indicates a reduction of the exit gradient by approximately half.Site work estimates consider the construction costs of internal cutoff levee and necessary road improvements for site access. The cutoff levee requires approximately 440,000 cubic yards of fill at $15 per cubic yard, therefore the total cost of the levee is approximately $6.6 million . For project access, revitalization of a portion of the former Victory Highway is the most likely option. The approximate length of road to be improved is 0.54 miles with an estimated cost of $1.8 million per mile constructed . This value reflects construction equivalent to a one-foot asphalt concrete layer and gives a total of approximately $972,000. An alternative route involving revitalization of a larger portion of Victor Highway stretching roughly 2.17 miles would cost $3.9 million, which is four times the cost of the first option. The total materials cost for the aquaponics system is approximately $23 million. This includes 600 aquaponics subsystems at $30,000 to $50,000 each, with an average of approximately $38,500. The prices of hydroponics and aquaculture components, including: tanks, pumps, anchors, and rafts per subsystem.

The prices are based on data from UVI Aquaponics, CropKing Hydroponics, Dock Float Supply, Dock Builders Supply and Seaflex Anchors. Labor and construction costs are not included as further design and bidding by licensed aquaponics contractors are needed. The cost of wetland development is extremely variable depending on the scale of the project and quality of labor and materials used. According to Dr. Alexander Horne, estimates for wetland construction range from $2,000 to $75,000 dollars per acre . For the purposes of this project, Dr. Horne recommends a value of $25,000 per acre of wetland constructed. Therefore for 700 acres of wetland restoration there is an initial cost of approximately $17-18 million. Operations & Maintenance The economic analysis of sturgeon production by Logan estimates costs of operations and maintenance per aquaculture system at approximately $3,500 to $4,000. This includes cost of labor, feed, electricity, and medicine. Assuming a 25 percent increase in these costs to account for agricultural labor, this results in operating and maintenance costs between $4,000 and $5,000 per subsystem. Consequently for the 600 systems proposed there is an annual cost of approximately $2.8 million. For the proposed system on Sherman Island, there are three chief sources of revenue. The first is the sale of agricultural yields from the hydroponics component. The second is the sale of fish yields from the aquaculture component. The third source is the economic value placed on various wetland services.

Income generated by agriculture is dependent upon the type of crop produced as well as the frequency of harvesting cycles. For the purposes of this model, it is assumed that average yield is approximately 11,000 pounds of crop per year per system. Price indices for crops typically used in aquaponic operations indicate that cilantro has the highest economic benefit of $88,000 per year, with onion having the lowest value of $1,100 per year. On average, each year of production will generate roughly $25,000 to $30,000 in revenue per subsystem, resulting in nearly $17 million of income over a two-year time period. The aquaculture component of this business model uses price indices developed for similar systems rearing sturgeon. Numbers generated from previous examples include considerations for 5, 10, and 15 brood stocks. There is a harvest time of 18 months for each system, and production will likely be staggered to produce maximum output and more continuous income for the project. Considering a time period of two years, fish production gives estimated revenue of roughly $17 million assuming a sale value of $4.5 per pound of sturgeon. Wetland value is based on a sum of averages of the following services: gas regulation, disturbance regulation, water regulation, water supply, waste treatment, habitat, food production, raw materials, recreation, and cultural ecosystem services . Revenue for these services is presented in Table 4.The wetland component of the system will provide carbon sequestration. Carbon sequestration is the capture of atmospheric CO2 by plants such as bulrushes and cattails through photosynthesis. The Bulrush wetland can grow approximately 500 g C/ m2 per year, half of which is preserved. The CO2 is absorbed by the plants and then incorporated into the soil biomass as the plants decay over time. A wetland can sequester as much as 25 metric tons of CO2 per acre per year. This helps to reduce carbon emissions while reversing subsidence . The system actively stops and reverses subsidence. By flooding the area with water the peat layer preserved because it is wet. As the plant biomass from the wetland decomposes it increase the peat layer and therefore reverses subsidence. Wetland vegetation and non-sellable crop yields from the hydroponics system can be used to restore peat. Similar subsidence reversal projects have been proposed and approve on and around Sherman Island. One such project includes Twitchell Island Ponds where an increase of 1.6 inches/year in addition to preventing subsidence of approximately 1 inch/year has been demonstrated. During flooding even there will be sediment will be transported into the system and then settle out before the water in release back into river. Therefore sediment can slowly build up with flooding events and also reverse subsidence .

In addition the wetland restores natural habitats for birds and aquatic species. The fish a reptile species that would take advantage of this wetland ecosystem include carp, crappie, striped bass, white catfish, and pond turtles. Along with hundreds of different bird species including the mallard duck, red-tailed hawk, eagle, Canadian goose, cinnamon teal, ring-necked pheasant, ruddy duck, wood duck and many more. The wetlands will serve to restore the delta wetland habitats as well as to sustain future bio-diversity of wetland species . Water contamination and waste is reduced by the aquaponics system because nutrients and water are recycled back into the system through water recirculation. This separation of the system from the surrounding environment reduces water waste due to runoff and agricultural pollution when compared to terrestrial agriculture. Terrestrial agriculture requires a large amount of water,ebb and flow the majority of which is not absorbed by plants but leaves the land via groundwater runoff. Agricultural runoff promotes algal blooms and eutrophication, which lower the oxygen concentration dissolved in the water and leads to death of aquatic species. The potential environmental concerns of the wetland include the production of methane from biomass decomposition. While wetlands sequester carbon and hence reduce carbon dioxide, they release other GHGs, primarily methane and nitrous oxide . The concern is that Methane has a Global warming potential 21 times that of CO2, and N2O has a GWP 310 times that of CO2. Most of the GWP of CH4 occurs in the first twelve to fifteen years after its release, whereas the GWP of CO2 lasts decadnto the water within the system and the transport of such toxins into the surrounding environment. The soil at Sherman Island has been exposed to pesticides, fertilizers, and dredged soils for the bay which were contaminated with heavy metals. Therefore because the proposed design is to partially flood the soil the toxins could leach from the soil. In order to mitigate this possible issue a test flood plot an monitor the toxin release levels. Leach test or Barrel tests can be used to mimic the conditions and will give results that can be used to assess further needs of mitigation. Additionally, the heavy metals such as the metals off of ships including copper, zinc, and nickel can be immobilized in wet peat soils.

Organic compounds will bind to peat and once the wetlands have been established the plants will also remove toxins from the water. Finally because the flood storage zone will contain a large volume of water the toxins will be diluted. There may be short term effects from pesticides so to prevent birds from being exposed to these toxins the use of bird scaring devices can be implemented until toxins diffuse. It is important to monitor the toxins before, during and after the installation of the wetland in order to understand the mitigation strategies that must be considered and implemented. Many of the environmental concerns can be monitored at demonstration sites including Mayberry Farms Subsidence Reversal and Carbon Sequestration Project and the Twitchell Island Ponds which both contain many of the same environmental considerations that will be faced in the flood storage zone. Fatty acid composition was determined in leaves of purslane plants exposed to saline treatments for 30 days. A sample of 50 g of fresh tissue leaves was dehydrated in a incubator at 60 ºC for 48 h. The dehydrated tissue was macerated and stored in 15 ml vials at room temperature, protected from light. An aliquot of 1 g of each sample was used to determine fatty acid composition, according with the procedures described by Liu, et al. . After hexane extraction, the samples were vortex-mixed, centrifuged, and the upper phase was collected prior to GC analysis. Samples were injected via an auto sampler onto a fused-silica capillary column in a HP 6890 gas chromatograph system fitted with a flame ionization detector and eluted with helium at 44.0 ± 1 ml/min, with a split ratio of 1:17. The injector and detector were heated to 250 ºC. The column was temperature programmed from 130 ºC to 180 ºC at 25 ºC/min, and then to 230 ºC at 2.5 ºC/min. Fatty acid methyl esters were identified by comparing GC retention times with those of a mixture of standard fatty acids methyl esters Mix C14-C22, . Fatty acids were quantified using peak areas integration against internal standard. In GL and GR cultivars, submitted to 30 days of saline treatment, the main fatty acids detected were C16:0, C18:3 and C18:2. Such high amounts of palmitic acid, has it was found in the P. oleracea leaves of control plants is unusual, previous studies reported an proportion of 17% of this fatty acid in the total FAMEs detected . Palmitic acid, the most abundant fatty acid in the human diet, causes oxidative DNA damage, DNA strand breakage, necrosis and apoptosis in human cells in vitro, but when consumed with others fatty acids, like PUFAs, is unlikely to have any significant impact on human health. The amount of unsaturated fatty acids was also a little bit lower than expected, although the proportions between UFAs and SFAs were maintained ; it has been reported in other studies that the linolenic acid content in P. oleracea leaves was circa 50% of total fatty acids and linoleic acid content 3- 4 times lower.

Wheat straw and sweet potato stalk were cut into about half to one inch pieces using a blender

However, only photosynthesis rates during leaf senescence of plant pre-cultured at low N supply reflected leaf senescence score during reproductive growth and N efficiency in the field experiments. Therefore, cultivar differences in leaf senescence during reproductive growth can only partly be reproduced in a short-term nutrient-solution experiment. Several differences between vegetative and reproductive growth might influence the induction and development of leaf senescence: first, although leaf senescence might be induced by N shortage both in hydroponics and under field conditions, the timing of N shortage is dependent upon different factors. In the field, the exploration of N sources in deeper soil layers might play the most important role for N uptake during reproductive growth . Thus in the field, root growth and morphology are the most important plant traits, which play only a minor role for N uptake in hydroponics. Secondly, source-sink relationships differ distinctly between vegetative and reproductive growth, both for carbohydrates and as a consequence also for N. The changes in assimilate flows might influence the development of leaf senescence, or at least the parameters used to characterize leaf senescence. However,potted blueberries the fact that photosynthesis rate during late stages of leaf senescence was significantly correlated to leaf senescence in the field experiments and to grain yield at limiting N supply suggests that cultivar differences in specific steps of leaf senescence related to the breakdown of the photosynthetic apparatus contribute to N efficiency in the field.

Life support systems are what make human travel a possibility. In long range space travels, such as the travel to Mars, life support cannot depend upon storage alone, it requires a fully regenerative system as well, i.e. waste must be reclaimed for reuse. Steam reformation, supercritical water oxidation, electrochemical oxidation, and incineration are a few of the solid waste reclamation technologies that are being developed and tested for use in space travel. Currently though, it seems that incineration might be the best choice among the previously mentioned, in providing a fully regenerative system. Through rapid conversion, incineration of the inedible parts of wastes and crops produces carbon dioxide, water, and minerals. Incineration is already the most thoroughly developed technology for use in a terrestrial environment. However, with the use of incineration in a closed environment, there is the eventual buildup of pollutants that are emitted in the process. The resulting NOx and SO2 pollutants need to be removed and recovered for reuse by a flue gas cleanup system. Important things to consider when developing a flue gas clean up technology for use in long range space travels are safety, energy requirements, sustainability, and doable under a micro-gravity condition. Due to the sensitivity and restrictions of space missions, a flue gas clean up system lacking in any of these considerations can be hazardous and could potentially compromise the missions. Technologies requiring things such as expendables or the use of catalysts are unsuitable for space missions due to the loss of valuable resources and the possibility of catalyst poisoning thus limiting the life-span of a catalyst. Also, due to the micro-gravity, it is difficult to use wet processes that handle liquids, such as spray absorbers.

Consequently, even though there are numerous flue gas clean up technologies developed , taking into consideration the limitation each provides, the number of reliable and applicable systems seem to be dwindling. Commercial activated carbon, made mostly from materials such as coconut shells and coal, has been studied for the adsorption and/or reduction of NOx and SO2 . In this paper, we study the use of the activated carbon prepared from hydroponic grown wheat straw and sweet potato stem for the control of air pollutants that are a result of incineration during space travel. Both wheat straw and sweet potato stalk are inedible biomass that can be continuously produced in the space vehicle. It was found that there is actually a minuscule amount of SO2 in the flue gas from the incineration of hydroponic biomass, and that most of the sulfur from the biomass ends up as sulfate in flyash. Since SO2 should have already reacted with the alkali metal, the technique entails the carbonization of the wheat straw and sweet potato stalk, resulting in an activated carbon for the adsorption of NOx and then a reduction of the adsorbed NOx by carbon to form N2. Since most NOx in flue gas from combustion is in the form of NO, and NO2 is readily adsorbed on the activated carbon, this paper concentrates on the removal of NO. Parametric studies on the production of activated carbon and the adsorption of NO by the carbon have been conducted. The optimal conditions and effectiveness of this procedure to regulate NO emissions have been determined.After the biomass was shredded, it was packed tightly into a stainless cylinder for pyrolysis and activation. Nitrogen and Carbon dioxide were used as the pyrolysis and activation gases, respectively. The gas flow rate for pyrolysis was 0.5-1.0L nitrogen per minute and activation was 0.25L carbon dioxide per minute.

The amount of biomass used was approximately 50.0-60.0g for each batch. Pyrolysis and activation temperature and times were varied during carbonization in order to determine optimal activation conditions. The activation temperature was 50°C higher than the pyrolysis temperature. The notation of the activated carbon “WS-2-600-1-650” and “SP-2-600-1-650” indicate that the activated carbon was prepared from wheat straw and sweet potato stalk, respectively with a 2 hrs pyrolysis time at 600°C followed by 1 hr of activation at 650°C. Once activation was complete, CO2 was supplied to the sample until it could be sealed in a container. Biomass on a space mission would likely come from a hydroponic system. The total mineral content of hydroponic biomass can be much higher than field-grown biomass if nutrients are supplied luxuriously to the hydroponic solution. A typical hydroponic plant solution has a nutrient ratio of N:K:Ca:P:S:Mg =16:6:4:2:1:1 and contains micro-nutrients of B, Mn, Zn, Cu, Co, Mo, and Fe. The concentration of potassium in the solution is about 6 mM. Thus, hydroponic activated carbon adsorption efficiencies may differ significantly from field-grown biomass. Field grown biomass generally has less than 10% mineral content, while hydroponic biomass can have as much as 30% mineral content. Because of this, many of the experiments were performed using char, which had been soaked in deionized water to remove the soluble minerals. After soaking, the char was drained and dried at 200o C for one hour before use. Specific surface areas of samples were determined by gas adsorption. An automated adsorption apparatus was employed for these measurements. Nitrogen adsorption/desorption was measured isothermally at -196o C. Before any such analysis, the sample was degassed at 250o C in a vacuum at about 10-3 torr. The nitrogen isotherms were analyzed by the BET equation, to determine the surface areas of the chars. BJH adsorption cumulative surface areas of pores of the samples also were determined.Most of NOx in flue gas from combustion is in the form of NO. Also, NO2 is readily adsorbed on the activated carbon. Consequently, efforts were directed to determine conditions for maximal removal efficiency of NO. Adsorption efficiency of NO on the activated carbon was studied to evaluate the effects of temperature, oxygen composition,square plastic pot moisture and flow rate on the production of effective activated carbon. The adsorption experiments were performed by using a simulate flue gas with variable concentrations of N2, carbon dioxide, oxygen, NO and H2O. NO and NO2 concentrations were analyzed by a chemilum inescent NOx analyzer . The amount of NOx absorbed by the activated carbon was determined from the difference in NOx concentration of the inlet and outlet gases. It was assumed that missing NOx was adsorbed by the activated carbon.Preparation of activated carbon was conducted by heating hydroponic grown wheat straw and sweet potato stalk under anaerobic conditions. Nitrogen and carbon dioxide were used as the pyrolysis and activating gases, respectively. Time and temperature were varied during pyrolysis and activation to determine optimal carbonization conditions. In order to determine optimal carbonization temperatures, samples of wheat straw and sweet potato stalk were heated at 100o C intervals between 300 to 800o C for two hours during pyrolysis and between 350°C to 850o C for one hour during activation. Afterwards, percent burn off was measured. Higher carbonization temperatures caused larger portions of the samples to burn off, as shown in Fig.2.

The burn off was 68% at 600°C and 86% at 800°C for wheat straw, while 66% at 600°C and 92%at 800°C for sweet potato stalk. Both samples appear to follow the same burn off trend, the percent burn off increases only slightly with the increase of temperature between 400°C to 600o C. However, pyrolysis and activation temperatures above 700o C were observed to cause significant amounts of wheat straw and sweet potato stalk to burn to ash. It is thus not recommended that reactions be run at temperatures exceeding 600o C. The decrease in surface area beyond 600°C is caused by sample burning off. Using temperatures much lower than 600o C would compromise the maximum amount of effective adsorption surface area attainable . It is not only important to run reactions at temperatures low enough to prevent burn off and ash formation but also high enough to generate effective surface areas, which would be at 600o C for wheat straw. Fig.4 shows the BJH adsorption cumulative pore area of wheat straw activated carbon generated at different pyrolysis and activation times. It is evident from the plot that samples derived from longer pyrolysis and carbonization times exhibited a higher micropore count compared to shorter times. As with temperature generation optimum, however, too long pyrolysis and activation reaction times cause an adverse increase in burn off percentage. Though wheat straw carbonized with a pyrolysis time of six hours and activation time of 2 hours still demonstrated a higher cumulative pore area than the shorter times, it also produces larger amounts of burn off. Since reaction temperatures were kept below 600o C, ash formation that would have diluted effective surface area was prevented. Even though ash does not form, pyrolysis and activation times must still be chosen to create a balance between pore formation and burn off, one that would generate a high micro-pore count but at the same time, minimize material loss. The optimal pyrolysis and activation times for wheat straw are 2 hours and 1 hour respectively.Optimal pyrolysis and activation temperatures and times for carbon preparation were determined based on the amount of NOx that can be adsorbed by the activated carbon. The adsorption capacities of wheat straw activated carbons generated by different pyrolysis and activation temperatures are shown in Fig.5. A gas mixture containing 250ppm of NO, 5% O2, 10% CO2, with N2 as the balance was passed, at a flow rate of 250ml/min, through a turbular reactor containing 2g of activation carbon at 25o C. It is evident from the plots that the WS-2-600-1-650 activated carbon had the best adsorption efficiency. Samples carbonized above 600o C have higher ash concentrations than those carbonized below, while those carbonized below have lower percent micro-pore counts and surface area—both explaining why wheat straw generated at 600o C had the best adsorption efficiency. The NO adsorption efficiencies of wheat straw samples carbonized by differing pyrolysis and activation times are shown in Figure 6. It is evident from the plots that activated carbons carbonized by prolonged pyrolysis and activation times have better adsorption efficiencies than those carbonized by shorter times due to higher pore count and BET surface area. The micro-pore count and the surface area of activated carbon increases with an increase of the preparation time, which explains why the samples with the longest pyrolysis and activation times have the best adsorption efficiencies. However, prolong activation results in more burn off and the production of ash. A balance must be reached when setting reaction parameters, one that will generate the largest surface area without a significant burn off. We have found that the optimal pyrolysis and activation times for wheat straw are two and one hour, respectively. The hydroponic biomass possesses high mineral content. The effect of the minerals on the activated carbon on NO adsorption efficiency was studied. The activated carbon was first soaked in water to dissolve the soluble minerals and then dried to remove the moisture from the carbon particles. The adsorption experiments using the mineral-free activated carbon were performed and the results indicate that the NO adsorption efficiency was substantially improved .

The lability of the sulfation modification has proven problematic for other MS/MS methods

This centrality-driven division between frontal and temporal semantic processing regions can be seen in the BOLD signal, with left IFG and left anterior MTG initially responding generally to the switch from non-word to word stimulus, before demonstrating clear correlation with central and peripheral HRF prediction peaks, respectively . While both temporal and frontal regions are implicated in semantic cognition, it has been suggested that left posterior MTG acts as a general interface between lexical and conceptual knowledge, anterior MTG is involved in specific semantic associations, while left IFG is more context specific, activating for conceptual knowledge that is cued by the preceding text . Consequently, for central textual ideas, which are more semantically-dependent on previous ideas, the IFG is increasingly involved in making appropriate semantic connections to the established context. On the other hand, processing peripheral ideas, or ideas which have looser semantic connections to the preceding text, would rely more heavily on regions that support general semantic knowledge to contextualize the present text. This suggests that within the fron to-temporal semantic control network, there is a functional divide between frontal and temporal contributions related to perception of textual centrality. Decreased activation over time for both central and peripheral ideas was similar to the patterns of temporal activations associated with passages—as language regions increased over time, activation of the visuospatial attention system decreased. This pattern is also apparent in the BOLD signal,chicken fodder system and appears to be anti-correlated with both central and peripheral phrases .

However, the extent and strength of the right IPS cluster in central ideas was significantly greater than peripheral. This difference can be explained by right IPS involvement in situation model construction—because central ideas contribute more to the situation model, they would consequently be more sensitive to the decreasing need of construction regions .Our temporal analyses assumed a linear relationship between time and neural activation of text processing; however, nonlinear temporal relationships may exist, and future studies should explore such non-linear changes. A second limitation is that our models assume that neural activation builds not only as the reader progresses through the paragraphs, but also during the baseline condition between the two paragraphs. Future work should compare whether removing this baseline assessment changes the patterns of temporal activation change. One methodological consideration is that our participants were skilled adult readers, and our passages were written at a fourth grade reading level. Future studies should manipulate the reading level of the passages and examine how this manipulation influences the neural correlates of expository comprehension, particularly regions associated with EF. Future studies should also consider the important interaction between text and reader by considering the background knowledge that the readers hold about each passage topic. Background knowledge plays an important role in building a coherent representation of the text and allows the reader to form a more meaningful representation that goes beyond the text-based ideas . A reader’s existing knowledge base is especially important to consider with respect to expository texts because they often use topic-relevant vocabulary that builds upon the reader’s assumed knowledge base.

Finally, future work should examine the neural correlates of building a coherent text representation among groups of readers known to be less sensitive to structural centrality, such as individuals with reading disability, individuals with ADHD, and foreign language learners. Comparing the patterns of activation associated with skilled and less skilled comprehension could help identify the comprehension processes that are disrupted and the underlying source of their comprehension difficulties. This insight could perhaps be employed to inform and improve reading comprehension instruction and interventions.Plasma membrane–localized receptors are critical components of the innate immune responses of animals and plants . Many of these receptors recognize and respond to the presence of conserved microbial molecules and are often referred to as pattern recognition receptors . In animals, this recognition is carried out, in part, by Toll-like receptors . Humans have 10 characterized TLRs that recognize conserved microbial molecules such as lipopolysaccharide or flagellin. In plants, cell surface receptor kinases and receptor-like proteins recognize microbial molecules in the apoplast . Well-characterized leucine-rich repeat –RKs include Arabidopsis FLS2 that detects flagellin, or its peptide epitope flg22, and the elongation factor Tu receptor that detects the bacterial elongation factor Tu, or its peptide epitope elf18 . Lacking an adaptive immune system, plants have an extended array of innate immune receptors encoded in their genome. Rice, for example, has more than 300 RKs predicted to serve as innate immune receptors based on the presence of a “non-RD” kinase domain, which lack the arginine-aspartate motif characteristic of most kinases . Of the few non-RD RKs characterized to date, all have a role in innate immunity or symbiosis . The rice XA21 RK, one of the first innate immune receptors isolated, mediates recognition of the Gram-negative bacterium Xanthomonas oryzae pv. oryzae , the causal agent of an agronomically important disease of rice .

Previous efforts to identify the microbial molecule that activates the XA21-mediated immune response led to the identification of a number of Xoo genes that are required for activation of XA21 . These genes encode a tyrosine sulfotransferase, RaxST, and three components of a predicted type 1 secretion system : a membrane fusion protein, RaxA; an adenosine triphosphate –binding cassette transporter, RaxB; and an outer membrane protein, RaxC. raxST, raxA, and raxB are located in a single operon . On the basis of these findings, we hypothesized that the activator of XA21-mediated immunity is a tyrosine-sulfated, type 1–secreted protein . However, the identity of this molecule has remained elusive . Here, we report the identification of the tyrosinesulfated protein RaxX as the activator of XA21-mediated immunity.In addition to raxST, we have previously identified two other rax genes involved in microbial sulfation. These genes, raxP and raxQ, encode an ATP sulfurylase and an adenosine 5′-phosphosulfate kinase, and work in concert to produce the universal sulfuryl group donor 3′-phosphoadenosine 5′-phosphosulfate . The requirement of these three genes for activation of XA21-mediated immunity by Xoo suggests that tyrosine sulfation plays a key functional role in this process. To further investigate this possibility, we transformed a raxST mutant strain , which forms long lesions on XA21-TP309, with a plasmid expressing raxST under control of its native promoter . PXO99DraxSTSp regained the ability to activate XA21-mediated immunity . RaxST carries a predicted PAPS binding motif conserved in mammalian sulfotransferases including the human tyrosine sulfotransferases TPST1 and TPST2 . In TPST2, mutation of the conserved arginine in the PAPS binding motif impairs enzymatic activity . We generated a similar mutation in raxST and tested if the expression of this mutant variant on a plasmid could complement the PXO99DraxSTSp infection phenotype on XA21-expressing rice plants. The strain PXO99DraxSTSp failed to activate XA21-mediated immunity , indicating that the sulfotransferase activity of RaxST is critical for its function. On the basis of the genetic association of raxX with the raxSTAB operon, the importance of tyrosine sulfation for activation of the XA21- mediated immune response, and the presence of a single tyrosine residue in PXO99 RaxX that is conserved among all available RaxX sequences , we hypothesized that RaxX Y41 is sulfated by RaxST and that this sulfation is required for RaxX function. To test this hypothesis, we transformed PXO99DraxX with a plasmid carrying a derivative of RaxX with tyrosine 41 mutated to phenylalanine [PXO99DraxX]. PXO99DraxX failed to activate XA21-mediated immunity in XA21-TP309 . We also demonstrated that sulfated RaxX peptides,fodder systems for cattle but not peptides carrying an Y41 to F substitution, are immunogenic on XA21-expressing rice plants . These results support the hypothesis that sulfation of RaxX Y41 is required for its activation of XA21-mediated immunity. To determine whether RaxX Y41 is sulfated by RaxST in vitro, we incubated a chemically synthesized peptide covering the C-terminal region of RaxX with purified His-RaxST in the presence of PAPS. Trypsin-digested peptides were analyzed by liquid chromatography–tandem mass spectrometry in both negative and positive nanoelectrospray modes with ultraviolet photodissociation to generate informative a, b, c, x, y, and z product ions from cleavage of the peptide backbone.

This method has previously been shown to facilitate the characterization of sulfated tyrosine residues within peptides by MS/MS.In negative ion mode, the sulfate group is retained on all product ions, thus allowing the sulfate modification to be unequivocally localized to Y41 of RaxX. MS/MS data showed fragment ions that account for 93% sequence coverage of peptide HVGGGDsYPPPGANPK . The high-resolution verification of the peptide mass in the negative mode MS1 is displayed in fig. S7. The extracted ion chromatograms of the peptides of interest and positive mode UVPD mass spectrum are shown in figs. S8 and S9, respectively. We next tested if RaxX is sulfated in vivo. Using selected reaction monitoring-MS , we observed sulfation on tryptic peptides covering Y41 derived from RaxX-His purified from PXO99 . The sulfated version of the tryptic peptide covering Y41 of RaxX-His purified from PXO99DraxST was not detectable with multiple SRM transitions above the background noise level . In contrast, we did detect the corresponding non-sulfated peptide covering Y41 at high levels and with high confidence for RaxX-His isolated from both PXO99 and PXO99DraxST . In combination, these results demonstrate that RaxST sulfates RaxX on Y41 in vivo and that sulfation of RaxX is required for its immunogenic activity on XA21-expressing rice plants.Infection assays using bacterial mutants clearly demonstrate that RaxX is required for activation of XA21-mediated immunity. We next sought to determine whether sulfated RaxX can trigger XA21-mediated defense responses in the absence of Xoo. For this purpose, we produced full length sulfated recombinant RaxX using an expanded genetic code approach . We heterologously expressed RaxX in E. coli together with an engineered aminoacyltRNA synthetase specific for sulfotyrosine, a cognate engineered amber suppressor tRNA, and the nonstandard amino acid sulfotyrosine . Nonsulfated RaxX was also expressed in E. coli without sulfotyrosine. We confirmed purity and tyrosine sulfation status of RaxX60 by gel-based assays and SRM-MS analysis . We tested if the resulting highly purified, full-length, sulfated RaxX60-sY and nonsulfated RaxX60-Y proteins could trigger defense gene expression in leaves of rice plants over expressing XA21 . As shown in fig. S13, sulfated RaxX60-sY, but not the nonsulfated form RaxX60-Y, triggered strong up-regulation of defense marker genes in detached leaves of Ubi::XA21. Leaves from Kitaake rice plants, which lack the XA21 immune receptor, are insensitive to RaxX60-Y and RaxX60-sY. These results demonstrate that sulfated RaxX60-sY is sufficient to activate XA21-mediated defense gene expression in rice. To identify a “minimal” epitope of RaxX that is sufficient to trigger these responses, we took biochemical and rational design approaches. We first tested whether chemically synthesized RaxX39 is sufficient to trigger XA21-dependent defense gene expression . We found that sulfated RaxX39-sY triggers defense gene expression in an XA21-dependent manner, whereas non-sulfated RaxX39 does not . To further narrow down the active region, we subjected RaxX39 to digestion with four site-specific proteases . The predicted digestion patterns were confirmed by gel-based assays and by SRM-MS analysis for ArgC, AspN, and trypsin digests . We tested the resulting RaxX fragments for their ability to activate XA21-dependent signaling . Only RaxX39-sY digestion products resulting from GluC and ArgC treatments retained activity on Ubi::XA21 plants. The ability of the ArgC fragment to activate XA21-mediated immunity was confirmed with chemically synthesized RaxX24-sY peptides . Next, we tested N- and C-terminaltruncated versions of RaxX24-sY peptides. Chemically synthesized RaxX21-sY retained the ability to induce XA21-dependent signaling, whereas RaxX18-sY was compromised in this activity . These results indicate that a chemically synthesized tyrosine-sulfated 21–amino acid derivative of RaxX, named RaxX21-sY, is sufficient to activate XA21- mediated defense responses. In addition to activation of defense marker gene expression, the activation of PRR-triggered immunity in plants often involves the production of ethylene and reactive oxygen species . These responses are known to contribute to the final disease outcome in many plant pathogen interactions . We therefore tested if RaxX21-sY can trigger these hallmarks of plant innate immune signaling in XA21- expressing rice leaves . As shown in Fig. 3 , only sulfated RaxX21-sY, but not RaxX21-Y, was able to activate defense marker gene expression and the production of ROS and ethylene in an XA21-dependent manner. These responses were most pronounced in rice plants overexpressing XA21 .

Plant activators are chemicals that have no direct antimicrobial activity but induce disease resistance

Plants with compromised SA synthesis or signaling have greatly diminished defenses against pathogens, as is the case with SA-defificient transgenic plants expressing a bacterial salicylate hydroxylase or ICS mutants like sid2 , and mutants in downstream targets of SA such as npr1 . SAR induction by biotic agents coincides with increases in SA levels and a systemic transcriptional reprograming that primes the plant to respond rapidly to minimize the spread or severity of further infections . This transcriptional reprograming includes the expression of pathogenesis-related genes and deployment of peroxidases and other defense factors. In addition to induction by biotic agents, SAR responses are induced by exogenous application of SA to the foliage or roots.A number of synthetic compounds have been developed that induce SAR by increasing SA accumulation and/or by acting on downstream targets of SA . For example, the plant activator, probenazole, effective against bacterial, fungal, and oomycete diseases, stimulates SAR by increasing SA levels . 1,2,3-Benzothiadiazole-7-thiocarboxylic acid-S-methyl-ester ,blueberry grow pot sold under the trade name, Actigard, stimulates SAR in many plant species without inducing SA accumulation . Tiadinil [TDL; N–4-methyl-1,2,3-thiadiazole-5-carboxamide] is a plant activator that was registered in Japan in 2003 under the trade name, V-GET. TDL was developed for disease management in rice where it is applied to nursery-grown seedlings for transplanting to production fields .

TDL is very effective for control of rice blast disease caused by Magnaporthe oryzae and appears to induce resistance in a manner similar to BTH by acting on downstream targets of SA . The TDL metabolite,4-methyl-1,2,3-thiadiazole-5-carboxylic acid, is responsible for the SAR activation . Abiotic stress alters the susceptibility of plants to many pathogens . The effect of brief episodes of root stress such as salinity and water deficit at levels that commonly occur in agriculture is well documented in plant–oomycete interactions, wherein stress events predispose plants to levels of inoculum they would normally resist . The phytohormone abscisic acid accumulates rapidly in roots and shoots as an adaptive response to these abiotic stresses, but also contributes to the increased disease proneness of the plants . Antagonism between SA and ABA is well documented in relation to plant defense responses to pathogens . Previously, ABA was found to have an antagonistic effect on SAR which was induced by 1,2-benzisothiazol-3-one1,1-dioxide and BTH in Arabidopsis and tobacco . However, it is not known if plant activators that target SA signaling impact the ABA-mediated susceptibility to root pathogens that occurs following predisposing root stress in tomato. Because of the potential for unwanted trade offs and signaling conflicts in plants exposed to different stresses, as can occur in the field, we investigated how predisposing root stress impacts chemically induced resistance in tomato. The objective of this study was to determine the effect of pre-treatment of tomato seedlings with TDL and BTH on salt-induced predisposition to the foliar bacterial pathogen Pseudomonas syringae pv. tomato and to the soilborne oomycete pathogen Phytophthora capsici. TDL is of particular interest in the context of soilborne pathogens such as Phytophthora capsici because it is often applied to plants as a root dip. We also determined the impact of SA, TDL and BTH on ABA accumulation during a predisposing episode of salt stress. The results show that TDL applied to roots strongly protects the leaves from disease caused by Pst in both non-stressed and salt-stressed plants.

In contrast, neither TDL nor BTH protects roots from Phytophthora capsici. The protection induced by plant activators against Pst does not result from reduced ABA accumulation and, although overall disease is less in both non-stressed and salt-stressed plants by chemically induced SAR, plant activators do not reverse the salt-induced increment in disease Severity.Tomato plants of cultivars “New Yorker” or “Rheinlands Ruhm” and mutants within these backgrounds were used in experiments. “New Yorker” seeds were obtained from a commercial source . The homozygous ABA-deficient mutant sitiens was compared with its isogenic, wild-type background, “Rheinlands Ruhm” , and seeds for these were obtained from the C.M. Rick Tomato Genetics Resource Center at the University of California, Davis. NahG transgenic plants were generated in the “New Yorker” background, similar to the method used by Gaffney et al. . The nahG construct containing the transgene salicylate hydroxylase under control of the CaMV 35S promoter in the binary vector pCIB200 was a gift of Syngenta Crop Protection, Inc. Tomato plants were grown in a hydroponic format. Prior to use, tomato seeds were surface sterilized with the following protocol: 50% HCl and rinsed with sterile deionized H2O, 10% trisodium phosphate and rinsed in sterile deionized H2O, 70% ethanol , and rinsed with sterile deionized H2O, and 50% commercial bleach followed by sterile deionized H2O rinse . Following surface-sterilization, seeds were placed on sterile germination paper in beakers containing sterile deionized H2O, transferred after 1 week to trimmed 5 ml polypropylene pipette tips, secured with foam test tube plugs, and placed into aerated hydroponic containers filled with 4 L of aerated, 0.5× Hoagland’s solution. Seedlings were grown for an additional 2 weeks in a growth chamber until at least two true leaves had developed on each plant.

To determine the effect of SA on ABA accumulation during salt stress, ABA levels were measured in WT plants pre-treated with SA, TDL, or BTH. Following salt stress treatment for 18 h, roots and shoots were collected and immediately frozen in liquid N2.The tissues were lyophilized and placed at −20◦C until extraction. The lyophilized tissue was ground in liquid N2 to a fine powder with a mortar and pestle, 50–100 mg samples were collected, and each sample transferred to a microfuge tube. Cold 80% methanol containing butylated hydroxytoluene at 10 μg ml−1 was added to each tube, which was then vortexed. The extracts were placed on ice and agitated occasionally for 30 min. The tubes were centrifuged for 5 min at 10,000 × g, and the supernatants collected. The pellet was extracted with 0.5 ml of 80% methanol and centrifuged to collect the supernatant. This step was repeated, all three supernatants were combined, and the methanol concentration of the extract adjusted to 70%. The extracts were applied to pre-wetted Sep-pak C18 columns and eluted with 5 ml of 70% methanol. The eluate containing ABA was concentrated to near dryness at 37◦C under vacuum and the volume adjusted to 300 μl with deionized water. The samples were analyzed by competitive immuno assay with an ABA immuno assay kit according to the manufacturer’s directions. Results are expressed as nanomoles of -ABA per gram dry weight of tissue. To determine the effect of the nahG transgene on ABA levels, roots and shoots from WT and NahG plants were processed using the same procedure as above.To determine the effect of the nahG transgene on SA accumulation following infection, SA was quantified in WT “New Yorker” and NahG backgrounds in non-inoculated plants and plants 3 dpi with Pst. Extraction of SA was carried out as previously described . Deuterated SA was used as an internal standard. Methyl ester derivatives were analyzed by GC-MS in electronic ionization mode. Mass spectral analysis was done in selective ion monitoring mode. Fragment ions were SA-ME 152 and SA-D4-ME 156. Quantification calibration curves were generated with known quantities of pure SA.To determine if plant activators induce resistance to Pst under different stress regimes in our experimental format,hydroponic bucket roots of hydroponically grown seedlings of cv. “New Yorker” were treated with TDL and then either not salt-stressed or exposed to 0.2 M NaCl for 18 h prior to inoculation. In preliminary experiments, several concentrations of TDL were evaluated for phytotoxicity and for efficacy against bacterial speck disease with 10 ppm TDL selected as this concentration provided an optimal response. Concentrations higher than 10 ppm of TDL caused a slight bronzing of the roots and depressed growth of the seedlings, suggesting a mild phytotoxicity of the chemical in our experimental format at these higher levels. Inoculated salt-stressed seedlings had more severe disease symptoms and a significantly higher titer of pathogen than non-stressed, inoculated plants. Pretreatment with TDL at 10 ppm significantly reduced Pst colonization and symptom severity in “New Yorker” plants in both non-stressed and salt-treated seedlings . However, TDL did not prevent the proportional increase in Pst colonization observed in salt-stressed plants relative to the non-stressed controls.Since TDL harnesses SA-mediated defenses, we treated SAdeficient NahG plants to see if TDL induces resistance under the different stress regimes in this highly susceptible background. As expected, NahG plants were more susceptible to Pst and accumulated significantly less SA following Pst infection than the WT background “New Yorker.” However, TDL provided strong protection in the NahG plants and mitigated the predisposing effect of salt-stress on bacterial speck disease.In a previous study we showed that ABA-deficient tomato mutants displayed a much reduced predisposition phenotype to salt stress . To determine if the protective effect of TDL is altered within an ABA-deficient tomato mutant, seedlings of WT and an ABA-deficient mutant within this background, sitiens, were treated in the same format and stress regimes as above. TDL significantly reduced Pst symptoms and colonization in both non-stressed and salt-treated plants of “Rheinlands Ruhm.” However, 3.6- and 5.4-fold increases in pathogen titer as a result of salt-stress were observed in both the control and TDL-treated plants, respectively, indicating that TDL did not prevent the proportional increase in Pst colonization in salt-stressed plants, similar to the results with “New Yorker” and NahG plants.

In contrast, the sitiens mutant was not predisposed to Pst by salt stress and had significantly reduced symptoms and colonization by the pathogen than the background “Rheinlands Ruhm” . Nonetheless, TDL pretreatment of sitiens provided further protection against Pst .To determine if plant activators protect tomato roots and crowns against the oomycete pathogen, Phytophthora capsici, and predisposing root stress, tomato seedlings were treated with TDL or BTH , not stressed or salt-stressed as above, and then inoculated. There was no protection provided by the plant activators against disease caused by Phytophthora capsici in either the control or salt-treated plants, as reflected in symptom severity and pathogen colonization .Because elevated levels of ABA in tomato can enhance susceptibility to Pst and Phytophthora capsici, the effect of SA, TDL, and BTH on ABA levels was determined in roots and shoots. ABA concentrations in either shoots or roots at the time selected for inoculation in our treatment sequence were not altered by SA . However, a trend of increasing ABA accumulation was observed in TDL- and BTH treated “New Yorker” plants relative to the corresponding control plants . Although the increase in ABA accumulation in the plants treated with these plant activators is not statistically significant at P ≤ 0.05, it can be said that SA, TDL, and BTH do not reduce ABA content relative to untreated plants . In addition, salt stress did not further increase the levels of ABA in plants that had been pretreated with TDL or BTH, which were similar to the salt stressed controls.In a previous study, we demonstrated the predisposing effect of salt stress and a role for ABA as a determinative factor in predisposition in the tomato–Phytophthora capsici interaction . The present study is the first report of salt-induced predisposition to the bacterial speck pathogen, Pst, in tomato. Furthermore, the results with the ABA-deficient sitiens mutant are consistent with the salt-induced susceptibility to Pst being mediated by ABA . These results conform to studies in Arabidopsis where ABA has been reported to promote susceptibility to Pst .Because SA has been shown to protect tomato against salt stress, possibly by an ABA-dependent mechanism , plant activators that operate via the SA pathway were evaluated for effect on salt-induced predisposition. Protection of tomato against bacterial speck disease by BTH is well documented , and TDL has previously been shown to reduce the severity of bacterial and fungal infections without inducing SA accumulation . Here, TDL was shown to protect against Pst in both non-stressed and salt-stressed tomato plants. TDL pretreatment strongly reduced disease and colonization by Pst in both “New Yorker” and SA-deficient NahG plants. TDL, or more likely its biologically active metabolite, SV-03, presumably allows the NahG plants to mount an SAR response to Pst infection in the absence of SA accumulation . TDL provided protection in both non-stressed and salt-stressed plants, but did not reverse the predisposing effect of salt stress.

This disturbs the Ca2þ flux in the plant cell, thereby resulting in ROS formation

We discuss nine in vivo studies that have used proteomics to explore the alterations in plants at the protein level, in response to metal-based NPs, including nAg, nCeO2, nAl, nAl2O3, nFe, nZnO and CdS QDs . Impact of bare or surface-functionalized nAg on plant proteomes have been studied in several plants, including arugula, wheat, rice, soybeans, and tobacco. In rice plants exposed to 30 and 60 mg/l nAg for 20 days in hydroponic growth media, 2DE-nanoLC/FTICR MS identified twenty-eight differentially-accumulated proteins, which were primarily involved in oxidative stress response, Ca2þ regulation and signaling, transcription, and protein synthesis/degradation, cell wall damage, and apoptosis . nAg exposure incremented levels of defense-related proteins including SOD, glutathione-S-transferase , L-ascorbate peroxidase, which has been shown to result from Agþ leaching. Similar defense response expressed by elevated levels of SOD and GST was reported in arugula and wheat plants, respectively, exposed to 10 mg/l polyvinyl pyrrolidone -coated nAg for 5 days . Comparative proteomic response in the roots of arugula plants exposed to nAg and AgNO3 suggested that both treatments disrupt redox regulation, biosynthesis of sulfur containing amino acids,nft hydroponic system and cellular homeostasis.However, nAg also alter endoplasmic reticulum and vacuole-associated proteins in arugula and wheat roots, not demonstrated in the Agþ treatments. Vannini et al. also reported an increased production of malate dehydrogenase in roots, which reportedly increased root exudation of organic acids such as citrate, oxalate, malate, succinate and acetate .

Zhao et al. demonstrated in a metabolomic study that uptake and translocation of nCu in cucumber plant triggers a feedback control mechanism in tandem to modulate the root exudation of amino acids and organic acids for defense response and restricting ion release, to maintain cellular homeostasis . In tobacco plants, a 7-day exposure to citrate-coated nAg in tobacco plants altered the proteins related to defense response and oxidative stress, at a similar level as AgNO3 treatments; however, although both nAg and AgNO3 affected mostly photosynthesis related proteins, in the leaves the effect was significantly higher in nAg exposed plants, highlighting a nano-specific response . In another study, proteomic analysis in soybean plants after 3-days of root exposure to three different metal nanoparticles showed significantly negative response to 5 ppm nAg treatments compared to 500 ppm nAl2O3 and 500 ppm nZnO . The drastic decrease in the proteins related to energy metabolism and a compromised defense system in the nAg exposed plants thus resulted in decreased growth of soybean plants, compared to the control, nAl2O3 and nZnO exposures. Proteomic analysis of soybean roots exposed to 200 mg/l of differentially coated-CdS-QDs in vermiculite showed over-accumulation and under-accumulation of 99 and 44 root proteins, respectively, irrespective of coating type . The response was also compared to bulk-equivalent and Cd2þ ion treatments. The affected proteins unique to QD exposures were involved in glycolysis, TCA cycle, urate oxidation, and ATP synthesis-coupled-proton transport. Stress signaling pathways were also upregulated, especially b-oxidation of fatty acids, biosynthesis of jasmonic acid and sphingosine, and lignin biosynthesis. Some proteins involved in defense response, ion binding, channel activity, and membrane organization were negatively affected. Ca2þ-transporting ATPase activity was also down regulated in all CdS-QD-treated roots. reported in nAg treatments as well.

Some altered proteins that were common to CdS bulk particles and Cd2þ ion exposure were those associated with pentose phosphate pathway, glucuronate pathway, Calvin and TCA cycle, glycolysis/gluconeogenesis, amino acid biosynthesis, catecholamine biosynthesis, GABA shunt, phenylpropanoid pathway, GSH metabolism, isoflavonoid synthesis, carbon fixation, glyoxylate/dicarboxylate metabolism, jasmonic acid biosynthesis, and terpenoid biosynthesis, and sucrose and starch catabolism.Although exploration of the plant proteome can deliver a wealth of information, the studies concerning ENMs have been primarily focused on toxicity. Tiwari et al. employed gel-based proteomics and transcriptomics to elucidate the mechanism of bio-transformation of KAuCl4 to nAu in 5-day old A. thaliana plants . A total of 10 and 15 spots from 2DE of root and shoot samples, respectively, were digested into peptides by trypsin, and analyzed using MADI-TOF-MS. nAu affected carbohydrate metabolism, electron transport chain and oxidative stress in plant tissues. The production of GSTs in A. thaliana shoots in response to increasing Au accumulation, suggested they play an important role in controlling oxidative stress during the reduction of Au ions to nAuNPs. A 14-day exposure to 250–1000 mg/l nCeO2 via foliar spray and root absorption resulted in significant effect on carbon fixation and energy production in pinto bean plants . This involved enhanced production of thylakoid proteins participating in photosynthesis, decreased production of RuBisCo and altered enzyme activities in the electron transport chain in mitochondria nCeO2 have shown to be very actively involved in the oxidative chemistry in plant cells, either acting as an antioxidant enzyme mimic or a stress elicitor .

In pinto beans, two key enzymes responsive to oxidative stress, ascorbate peroxidase and glutathione peroxidase were down-accumulated. Altered response of ascorbate peroxidase enzymes has also been reported at biochemical level in kidney beans and tomato , as well as at transcriptomics level in A. thaliana . Interestingly, transcription factors , which play a central role in protein biosynthesis and turnover were shown to be down regulated in the leaves as well as first generation seeds of bean plants exposed to 125–500 mg/kg nCeO2 . Lipoxygenase, a protein responsible for fatty acid biosynthesis, iron binding, and oxido-reductase activity was also down-accumulated in bean leaves and seeds . The differentially regulated proteins in the seeds obtained from nCeO2 treated parent plants were mostly down-accumulated compared to those harvested from untreated controls, and a dose-dependent increase in the number of candidates were noted. The candidate proteins were involved primarily in storage , carbohydrate metabolism , protein folding, and resistance mechanism .Recent advances in tools for genomics and transcriptomics in conjunction with metabolomics and proteomics have the potential to accelerate agricultural development . An area that can benefit substantially from these approaches is the plant-microbe interaction. Microbial communities play an important role in plant growth and productivity by directly controlling soil processes like stabilizing soil structure, nutrient bio-availability, degradation of organic pollutants, CO2 fixation and C degradation. They are however highly sensitive and susceptible to toxicity from stressors. Next generation sequencing technologies such as pyrosequencing and Illumina-based sequencing has resolved complexities in the microbial community with higher accuracy than conventional methods . Metagenomics allows to collectively characterize genome sequences of known and unknown members of the entire microbial community in an environmental sample, without having to isolate each into pure cultures. ENMs present in the agricultural soil from intentional use of nano-enabled agrochemicals or unintentional incorporation in the biosolids or irrigation water have the potential to impact the soil microbial community thereby affecting the agricultural productivity . Metagenomic analysis of the soil microbial community provide potential means to design sustainable ENMs with potential to bolster plant protection against pests and enhance productivity and nutritional quality . However, although the metagenomic analysis provide important information on the functional capacity of the soil microbial community, it does not reflect the metabolic activities of individual species or the community. Genomic interpretation can be complemented with gene expression analysis at the RNA level, also known as transcriptomics, which can provide critical information on sustainable ENM design for agricultural applications and impact on plants and soil microbial community. Advances in gene expression analysis using RNA-seq analysis have evolved the understanding of alteration in gene expression in complex samples, which can be further validated by RT-PCR analysis of targeted genes. The transcriptomic studies in plants exposed to ENMs have been very well reviewed in two reviews, where the authors discussed the integration of transcriptomics, micro-RNA and proteomics data in plants for discovering nano-specific biomarkers and effects . For transcriptomic studies, the knowledge of the whole genome sequence and their functional annotation is critical and hence is better addressed in smaller genomes. A. thaliana has the smallest genome that is completely annotated unlike the crop species like rice or soybeans ,hydroponic nft system and hence all global transcriptome studies focusing on ENM-plant interactions have been carried out in A. thaliana to avoid complexity .

However, complementary proteomics and metabolomics can be used to functionally annotate the genes of interest in non-model species. In theirreview, Ruotolo et al. concluded that the plants induce defense mechanisms against ENM exposure which are resolved at the transcriptome and proteome level and are primarily related to modification of root architecture, phytohormone signaling and antioxidant system activation .Integration of multiomics in plants provides a comprehensive knowledge on the regulatory mechanism at multiple subcellular organization levels in response to an external stimulus. Although the number of plant studies employing individual omic techniques to identify key biomolecules in response to ENMs is increasing, only a few studies have integrated different omics . It is critical to integrate the response at all levels, including metabolome, proteome and transcriptome to identify the molecular underpinnings that regulate the metabolic pathways in response to ENMs, which eventually is expressed in the phenotype. However, there are multiple challenges that have slowed down the use of integrative approaches, especially in crop species. The first challenge is the unavailability of transcriptomic databases and incomplete or un-annotated proteomic databases for non-model species. The second bottleneck is the difficulty in scaling very large datasets from different levels within the phenome. Being closest to the phenotype, the metabolome is easily influenced by immediate environmental conditions, which may or may not correlate with genomic and transcriptomic profile. Hence, the emergent properties at the higherlevel organization of the plant are not fully determined by the properties of the lower levels . Thirdly, metabolomic analysis is often oversimplified due to the common assumption that the sampling is performed in metabolic steady-state, characterized by constant levels of metabolites and that different metabolic pathways operate in isolation. At the whole plant level of integration, there are too many complex and dynamic processes occurring simultaneously which regulate feedback mechanisms in the cellular metabolism. In order to integrate the data from different omics in a test specimen, it is necessary to use the same sample for aliquoting into fractions for individual omic analysis. Assuming that the data acquired from each omic is of high quality and validated, they can be integrated by different approach, including postanalysis data integration; integrated data analysis; and systems modeling methods . In postanalysis data integration, the omic datasets are analyzed in isolation and the key features are networked in an overall model pathway. In integrated data analysis,specialized tools are used to merge different omics data sets prior to data analysis and interpretation. Systems modeling approaches incorporate modeling tools utilizing preexisting comprehensive omic databases . Only a handful of studies have utilized integrative approach in ENM-plant interaction studies and have relied primarily on postanalysis data integration . Integration of the plant metabolome with the proteome and transcriptome can answer several questions regarding ENM-associated mechanisms, including routes of entry, translocation, defense response and toxicity . Individual omic studies across different plant species and postanalysis integration studies over the past seven years have highlighted a few metabolic pathways of interest, depending on the mode of exposure. In root exposure studies, ENMs have shown to induce oxidative stress in the root tissues due to the immediate availability of metal ions from ENM dissolution, which is appreciated by the release of acidic exudates . As a defense response to ENMs, multiple metabolic pathways have been reported to be differentially regulated, including glutathione metabolism, GABA shunt, phenylpropanoid pathway, shikimate pathway, and flavonoid pathway. Different phenolic compounds and amino acids are evidently altered in these pathways in order to defend the plants from reactive oxygen species . Another common sign of stress in the plant roots is an increase in lignin content and alteration of membrane lipids, which protect the roots from additional stress . In several studies, nCu and CdS-QDs exposure altered levels of Ca-binding proteins, such as calmodulin or Ca2þ-ATPase . It is hypothesized that ENMs often bind to Ca2þ receptors or use the Ca-channels or Ca2þ-ATP pumps to enter the plants.The glutathione pathway and the GABA shunt play a major role in ROS defense in plants affected by exposure to ENMs. In addition to defense responses in different plant tissues, ENMs have been shown to influence carbon fixation, amino acid metabolism, and photosynthesis in the aerial tissues. In a recent study, metabolomics of leaf mesophyll protoplast showed that nFe and Mn3O4 enhanced the photosynthetic quantum yield . However nMoS2 and nAg had negative effects .

The linear approximation strategy is not without limitations

In a stylized story of green revolutions, improvements in agricultural technology are achieved through the introduction of improved land management techniques or improved inputs, including germplasm and fertilizer, all of which boost yields and labor productivity . If food is relatively non-tradable beyond local markets, then increased staple food production leads to reduced food prices, increased real wages and hence lower poverty. As staple yields jump and basic food needs are met, crop production begins to diversify, including to nonfood cash crops for export, and so the virtuous cycle of commercial farming begins. With greater savings and access to finance, farms begin to substitute capital for labor, and freed up workers begin to look for wage employment, typically in nearby cities. To the extent that other sectors enjoy higher labor productivity, this is welfare enhancing. It is also possible that this structural change triggers even further increases in non-agricultural labor productivity. One potential mechanism is that after subsistence is surpassed, savings rates increase, and the subsequent capital accumulation increases worker productivity . In parallel, governments are able to collect revenues to finance growth enhancing infrastructure, such as roads and ports, which increases the worker productivity of manufacturing and services. Another mechanism may be that increased incomes improve health outcomes,flood and drain tray which increase worker productivity, while also decreasing child mortality, reducing total fertility rates, increasing investment per child, and decreasing demographic pressures.

Or, it may simply be that the non-agricultural sector enjoys increasing returns to scale due to fixed costs or learning-by-doing, which would imply that a green revolution and the resulting labor shift would accelerate productivity growth in these non-agricultural sectors. Although our paper will not be able to pinpoint which of these mechanisms is at work, our contribution is to provide a causal framework to evaluate whether higher staple yields trigger labor shifts away from agriculture as well as faster growth in non-agricultural labor productivity. For the purposes of illustration and to motivate our empirical work more specifically, we describe agriculture-driven structural change with a simple model following the long theoretical tradition starting with works including Rostow ; Johnston and Mellor and formulated mathematically by Laitner ; Hansen and Prescott ; Gollin et al. , and others. We start with a country that has no trade in staple food products, and where the entire population works in either the agriculture or non-agriculture sector . The model is dynamic, but we dispense with the time subscript for simplicity of exposition. The stylized facts support the theoretical link between staple crop yields link and economic growth. Figure 1 shows indexed regional trends in food production per capita across the developing world from 1961-2001.The graph highlights the major growth in East Asia and the Pacific over the period, with per capita values nearly doubling, and considerable growth in Latin America and South Asia since the mid-1970s. Africa is the one region to have experienced a decline in per capita food production over the period, including a major decrease since the early 1970s and relative stagnation since 1980. These trends are mirrored in Figure 2, which presents cereal yields per hectare from 1961-2001. Again, all developing regions except Africa experienced major sustained growth rates in land productivity over the period, despite varying starting points, and all except Africa more than doubled yields by 2001.

East and Southeast Asia boosted yields from less than 1.5 tons per hectare in 1961 to more than 4 t/ha in 2001; Latin America’s yields grew from 1.3 t/ha to greater than 3 t/ha; and South Asia’s from 1 t/ha to nearly 2.5 t/ha. Africa had the lowest starting point at 0.8 t/ha, and still after 40 years had barely crossed the threshold of 1 t/ha, which was South Asia’s starting level in 1961. A simple Boserup hypothesis would argue that, relative to other regions, Africa’s yield stagnation is a product of its land abundance, and yields will increase as land becomes scarce. There are three main reasons why this hypothesis does not hold, as described in McArthur . First, the history of 20th century yield take-offs in the developing world was predominantly characterized by proactive public policies supporting a package of yield-boosting inputs, rather than by factor scarcity . These policies are thought to explain much of the regional variations in fertilizer use since 1960, as shown in Figure 3. Second, labor/land ratios vary tremendously across Africa but they are just as high or higher in many African countries than they were in pre-green revolution Asian countries. Third, land productivity is driven by the crucial latent variable of soil nutrients, which are being depleted at dramatic rates throughout Africa. High rates of soil nutrient loss strongly suggest that land pressures are not being surmounted by extensification.Figure 4 compares the growth of cereal yields to growth in GDP per capita over the 1965 to 2001 period, indicating a strong positive correlation between the two variables. A novel relationship is presented in Figures 5 and 6, which compare initial cereal yield levels to subsequent GDP growth across developing countries, excluding fuel exporters and socialist economies.Figure 5 covers the full 1965 to 2001 period and Figure 6 covers only the latter portion from 1985 to 2001. The horizontal line marks zero average growth and the vertical line marks 2 t/ha of cereal yields. In addition to the overall positive relationship between initial yield and economic growth, it is noteworthy that no country in the sample experienced negative average growth after reaching a yield threshold of 2 t/ha.3 Figure 7 presents a scatter plot similar to Figure 4 but shows growth in non-agricultural value added per non-agricultural worker on the vertical axis instead of GDP per capita, covering the period 1970-2001.

The graph shows a clearly positive relationship between the two variables, even amidst a considerable degree of variation, and suggests that higher rates of progress in agricultural productivity are structurally correlated with higher growth rates in non-agricultural sectors.This paper’s empirical strategy proceeds in two parts. The first focuses on establishing a country-level physical production function for cereal yields , in order to motivate the emphasis on agronomic inputs in a study of structural change. The second part focuses on identifying the impact of increased yields on economic outcomes and structural change, measured by GDP per capita, labor shares and non-agricultural value added per worker.It was chosen over log-linear and log-log approaches since neither of the latter were found to provide a better fit with the data, and indeed most countries with significant input use have pursued relatively linear fertilizer-yield trajectories, as shown in Figure 8. This linear relationship is somewhat at odds with the field-level agronomic data that show decreasing returns, but is likely an inherently limited product of the country-level unit of aggregation. This paper aims to present a first approximation of a country-level agricultural production function, which to our knowledge has not been previously done in the economics literature. Future research would be well placed to provide more refined estimates anchored in more specific crop types and input combinations, the latter captured for example through a range of possible interaction terms. With these points in mind, this paper’s regression results provide information only on marginal additive effects of various inputs. One might hesitate to interpret associations between agronomic inputs and yields in a causal framework; indeed, omitted variables such as farmers’ agronomic know-how might be correlated with both yields and inputs and thus bias coefficients in the estimation. In order to assuage these concerns and improve identification in the case of fertilizer use, we construct a novel time-varying instrument. Our approach follows a similar spirit to the instrument presented in Werker et al. . A valid instrument needs to be correlated with countries’ fertilizer use and satisfy the exclusion restriction . We use fluctuations in the global fertilizer price to generate temporal variation exogenous to conditions in any one developing country. In order to generate the cross-sectional variation in the instrument we exploit the fact that the production of nitrogen fertilizer is intensive in natural gas usage and therefore produced in only a select group of facilities around the world, most of which are in developed countries. We contend that the distance fertilizer travels from these facilities to the agricultural heartlands of each developing country is valid cross-sectional variation that can be interacted with the global fertilizer price to generate a valid instrument for fertilizer use in developing countries. Specifically, we hypothesize that countries closer to fertilizer plants are more sensitive to the commodity’s price variation relative to the transport costs that farmers incur. The instrument satisfies reverse causality concerns ,nft hydroponic and the omitted variable bias concern is assuaged since a problematic omitted variable would need be to correlated with the global fertilizer price and have the same distance decay function from agricultural heartlands to global fertilizer production facilities. A specific concern that a reader might have is that fertilizer price fluctuations might be correlated to fossil fuel prices, which might affect economic outcomes through many channels. However, the correlations between crude oil prices and phosphate, DAP, urea and potash prices are only between 0.11 and 0.38 over the period . Moreover, the correlation is only problematic if the specific distance decay function we use from agricultural centroids to nitrogen facilities matches the pattern of cross-country differences in fossil fuel prices, and there is no reason to believe that this will be the case. We use a Geographic Information System to calculate the agriculturally weighted centroid of each country, using data on percentage of each 5 arc-minute grid cell’s area planted to staple crops from Monfreda et al. . Next, we geolocate 63 of the production facilities of the top fertilizer producers in the world .

Although these are present-day facilities , we remind the reader that most facilities are located in developed countries not in our sample, and many locate in proximity to natural gas deposits, so the issue is unlikely to have a big effect on our results. We then calculated the minimum cost adjusted distance from each country’s agriculturally weighted centroid to the nearest fertilizer production site. In order to adjust for relative transport cost between land and water, we use Limão and Venables’ result that shipping a standard 40-foot container from Baltimore to different destinations around the world in 1990 costs $190 for an extra 1,000 km by sea and $1,380 for an extra 1,000 km by land. This indicates roughly a 1:7 cost ratio, which we use to optimize travel over sea and navigable rivers versus travel over land. The centroids, fertilizer production sites and optimal cost-distance function are mapped in Figure 9. The distance component of the instrumental variable is itself strongly correlated with fertilizer use across countries, as shown in Figure 10, which plots the log of fertilizer use per hectare at the 1985 sample midpoint against the indexed distance measure. The correlation between the two variables in the graph is -0.63. Towards the top left of the scatter plot, a country like Vietnam has an distance index value of 3,954 and a fertilizer value of 84 kg/ha, while Rwanda , towards the bottom right, has a distance value of 13,083 and a fertilizer value of 1.7 kg/ha. It is trivial for higher agricultural productivity to be linked to higher economic growth in the same period, since agricultural output is included directly in national accounts. For example, if one holds fixed all prices and production levels in other sectors, a green revolution-style five-year doubling of output in a low-income country with 30 percent of GDP in food production would translate mechanically to a 5.4 percent annual real GDP growth rate.For a country with only 15 percent of GDP in food production, the same yield doubling would translate to 2.8 percent annual growth. Of course a major supply expansion would be expected to decrease the price of food, and the nominal measured growth rate would be much smaller—so 5 or 6 percent could be considered an upper bound on the direct contribution of increasing yields to economic growth.

African-American groups have sought to reclaim and remold their rich heritage through urban farming

We refer to a farming system as “diversified” when it intentionally includes functional biodiversity at multiple spatial and/or temporal scales, through practices developed via traditional and/or agroecological scientific knowledge. Farmers manage this functional biodiversity to generate critical ecosystem services to agriculture . At the plot scale, diversified farming systems may include multiple genetic varieties of a given crop and/or multiple crops grown together as polycultures, and may stimulate biodiversity within the soil through addition of compost or manure . By crops, we mean either annual or perennial crops, including tree crops. At the field scale, DFS may include polycultures, non-crop plantings such as insectary strips, integration of livestock or fish with crops , and/or rotation of crops or livestock over time, including cover cropping and rotational grazing. Around the field, DFS may incorporate non-crop plantings on field borders such as living fences and hedgerows. At the landscape scale, DFS may include natural or semi-natural communities of plants and animals within the cropped landscape/region,4x8ft rolling benches such as fallow fields, riparian buffers, pastures, meadows, woodlots, ponds, marshes, streams, rivers, and lakes, or combinations thereof . The resulting heterogeneous landscapes support both desired components of biodiversity and “associated biodiversity”; together these two elements make up agrobiodiversity .

Components of the agrobiodiversity within DFS interact with one another and/or the physical environment to supply critical ecosystem services to the farming process, such as soil building, nitrogen fixation, nutrient cycling, water infiltration, pest or disease suppression, and pollination, thereby achieving a more sustainable form of agriculture that relies primarily upon inputs generated and regenerated within the agroecosystem, rather than primarily on external, often nonrenewable, inputs . Spatial considerations are important, since different components of the system must be in sufficient proximity, at each relevant scale, to create needed interactions and synergies. For example, the utility of intercropping for reducing below ground soil disease depends on spacing the different crops such that their root systems interact . Similarly, wild bee communities can only provide complete crop pollination services when a sufficient proportion of their natural habitat occurs within a given distance of crop fields . A DFS is not only spatially heterogeneous, but is variable across time, due both to human actions , and natural successional processes. Figure 1 presents the conceptual model of a DFS. The term agroecology goes back more than 80 years and originally referred to the ecological study of agricultural systems . Much agroecological work seeks to bring Western scientific knowledge into respectful dialogue with the local and indigenous knowledge that farmers use in managing ecological processes in existing agroecosystems . More recently this hybrid science has evolved to include the social and economic dimensions of food systems .

Partly in response to the industrialized agriculture of the Green Revolution , agroecology also came to mean the adoption of sustainable agricultural practices , and became an integral component of various social movements seeking alternatives to industrial agri-food systems. Thus agroecology currently holds multiple meanings, and can refer to an inter- or transdisciplinary science, a set of sustainable farming practices, and/or a social movement . DFS is not an alternative to agroecology. Rather, DFS is a framework that draws from agroecological, social, and conservation sciences to focus analytical and action-oriented attention toward farming systems in which cross-scale ecological diversification is a major mechanism for generating and regenerating ecosystem services and supplying critical inputs to farming. Agroecological principles and methods can be used to evaluate DFS and to design or revive processes of diversification . In this essay and series of articles, we explore the ramifications of DFS for both ecological health and socioeconomic welfare, as well as examining the intersection of DFS with existing industrialized agricultural systems, supply chains, and national and international policies. DFS are complex social-ecological systems that enable ecological diversification through the social institutions, practices, and governance processes that collectively manage food production and biodiversity . As many political ecology scholars emphasize, ecosystems are densely interconnected with social relationships . Ecological variables such as soil, water, and habitat help configure an array of farming practices, exchanges of food and resources, and landscape management decisions that, in turn, influence the structure and function of the ecosystem. Further, as ecosystem services are generated and regenerated within a DFS, the resulting social benefits in turn support the maintenance of the DFS, enhancing its ability to provision these services sustainably . This interplay underlies numerous historically occurring and emerging DFS worldwide. Conversely, socio-political and economic processes such as the decrease of access and control over seeds or increased dependence on commodity markets can intervene to disrupt such feedback cycles, thus weakening DFS. The industrialization of agriculture has led to growing homogeneity across food systems as farming techniques and markets become more standardized .

As a consequence, the complex social relationships underlying agriculture and ecosystem service provision have become less visible. Focusing on DFS can help farming communities, researchers, policy makers, and industry recognize and restore these relationships. At their core, DFS depend on agroecological principles that are developed in and through the social relationships among working farmers, their communities and environments, and researchers, including ecologists, anthropologists, agronomists, and ethnobiologists . As seen in the Kreman et al. examples these principles take varied forms depending on local conditions. To understand how DFS may develop, function, and evolve over time and space, the particular context of each DFS needs to be studied, paying particular attention to the politics and power relations that reciprocally shape its ecological conditions. Many DFS were developed through traditional and indigenous farming knowledge and agrobiodiversity that was accumulated over millennia . More recently, other DFS have been created through targeted agroecological studies designed by scientists to solve particular problems . Historically, much knowledge about biologically diverse farming practices has been created and shared through peer-to-peer learning within traditional farming communities and, more recently, also through their collaboration with researchers interested in further developing agroecology . These relationships continue to be critical to the growth of DFS in new societal contexts and geographic locations. Since the 1980s, with the rise of the Campesino-a-Campesino and La Via Campesina movements, institutions such as government agencies, domestic and international NGOs, and universities have become increasingly active in promoting and diffusing agroecological principles through research networks and programs . These actors have added new institutional dimensions to the social relationships that help sustain DFS. An illustration of how social and ecological systems interpenetrate within DFS is in the Andean highlands, where indigenous farmers have managed their lands agroecologically for 3,000 years . The ongoing interplay between human management and physical ecology has created a landscape of agroclimatic belts at different altitudes, each characterized by specific field rotation practices, terraces, and irrigation systems, and the selection of specific animals, crops, and crop varieties . Within these belts, traditional knowledge has helped sustain tremendous genetic diversity, by perpetuating adapted land races and wild relatives of crops. Social cooperation is essential to managing the verticality and heterogeneity of the Andean ecosystem. A barter economy based on reciprocity, for example, facilitated complementary exchanges of plants and animals between ecological zones along the steep elevation gradient . In industrialized systems in both developed and developing countries, farmers must now negotiate with corporate food buyers, buy agrochemical and seed inputs from agents, seek loans from bank officials, and work with agricultural extension experts trained in pesticide use. Farmers rely on such relationships to compete effectively in supply chains and to manage changing ecological conditions, such as pest outbreaks. Nonetheless, these particular types of relationships often push individual farms to increased dependence on banks, damaging livelihoods, and undermining collaborative social learning groups as farmers specialize in a single crop and maximize short-term yields through the use of external inputs, to meet loan repayments.

The economic pressures in these tightly linked systems generally corrode ecosystem services, which are the very foundation of support for potential DFS. Farmers in industrialized systems may also engage in exploitative relations with immigrant or impoverished laborers, paying inadequate wages and enforcing long hours,flood and drain table helping perpetuate the apparent cheapness of food. Industrial production creates a number of “distances” between producers and consumers such that information flow diminishes across the supply chain . Thus within the industrial agri-food system, consumers remain relatively ignorant about the conditions of production, and would be less able to choose between products based on sustainability criteria, if they value these, and to exercise their buying power in favor of DFS. In turn, the risk perceptions of consumers and corporations may inhibit the growth of DFS. For example, during the recent food safety scare in fresh leafy vegetables in California, corporate buyers insisted that growers remove native vegetation bordering fields that might attract wildlife. This action was taken largely to assuage consumer concerns, despite the lack of scientific support . In alternative agricultural systems such as organic or low-input farming, farmers can build particular forms of relationships that help sustain ecosystem services and social infrastructure more effectively. We discuss many of these relationships, including direct marketing, fair trade certification, and food justice movements. In developing and studying these alternative systems, however, researchers, policy makers, and NGOs often neglect race, socioeconomic, and gender issues, or sublimate them into a broad social justice category. Finding ways to be far more inclusive of diverse racial, gender, and socioeconomic groups can help strengthen the socialecological basis of agriculture. For instance, African-American growers once represented a sizable proportion of the U. S. farmer population, or one million in 1910, declining to 18,400 by 1997, due to race discrimination and violence, lack of land tenure , and multiple waves of economic migration from the South to urban centers . Many of these black farmers used DFS practices; their displacement helped create an opening for industrialized monocultures. Now, many new farmers in rural and urban areas are black, Latino, or Asian; there is evidence that these farmers are more likely than their established peers to embrace sustainable agriculture practices if adequately supported . Immigrants such as the Hmong may sometimes develop culturally relevant, more diversified food production enclaves within industrialized systems that preserve their traditions and provide livelihoods.They are developing new linkages between cities and nearby rural areas, potentially helping recreate DFS. For example, Will Allen founded Growing Power, an urban farming NGO that serves disadvantaged neighborhoods in Milwaukee and Chicago, attempting to encourage youth of all races to take up diversified farming. In Chicago, black activists and physicians have formed the Healthy Food Hub, a food aggregation NGO which sources produce from a historically black farming community, Pembroke Township, about an hour from Chicago. These efforts show how people can demand greater political agency in building a democratic DFS . New quantitative and qualitative research is badly needed to evaluate and critique the social benefits that DFS may provide in contrast to industrialized systems. In general, further analysis is needed to understand how the social elements of DFS can help generate and regenerate ecosystem services, thus maintaining diversified farming systems. In turn, more research is required on the political and socioeconomic interventions that could help rebuild or sustain the socialecological cycles that underlie DFS.DFS are often embedded in social, political, and economic conditions that differ from those accompanying industrialized monocultures , particularly with respect to core stakeholders, markets, and distribution systems. Yet, DFS may not always be able to realize their potential social-ecological benefits due to the lack of enabling environments. We explore how alternative agri-food networks and social movements relate to DFS and assess their potential to both maximize social benefits and promote DFS through their demands for food sovereignty and food justice. The agri-food systems approach reveals the interconnected systems of inputs, labor, land, capital, governance and knowledge that maintain specific types of agricultural production, distribution, and consumption systems . The governance and structure of the food system upstream from the farm, such as international agricultural trade liberalization policies that promote cheap food imports from industrial into developing countries, government subsidies for fossil fuel-based agrochemicals and commodity crops and irrigation projects that primarily benefit larger landholders , all help to maintain the industrialized agri-food system .

Five research groups have succeeded in coupling aggregate crop into climate models

Coupling with landscape microclimate models provides not only the vertical inputs used by agricultural models, but also gradients along the landscape. Coupling with hydrological models provides water flow paths such as surface runoff, vertical and lateral groundwater flow, and interactions between shallow soil and groundwater zones and with adjacent surface water bodies . Water quality models provide sediment and solute transport along the landscape controlled by water flows, and other effects such as wind erosion. Integration and upscaling of landscapes into the watershed scale requires three-dimensional coupling of the surface and subsurface water, energy and mass transfers. Condon and Maxwell and Maxwell et al. provide more details on coupled versus integrated models. At this scale, the groundwater aquifer system typically transcends the boundaries of the watershed, necessitating regional scale analysis to evaluate not only the impacts of cropping and animal production systems on water quantity and quality, but also feed backs from the hydrological system into the agricultural system . Further, meso-scale rainfall and evapotranspiration distribution models control the local surface and subsurface flow intensities, pollution and abatement . At this scale, human effects through land use changes, and ecological dynamics and transitions on natural or protected lands are also important components needed to evaluate overall sustainability of agricultural systems .Although some efforts have gone into integrating biophysical models ,mobile vertical rack more is needed to enable comprehensive assessments of agricultural systems across scales and adequately address environmental and economic responses to decisions and policies.

The need to address decisions and policies at scales arises frequently in agricultural system modeling. Resolving the time and space scale differences among model components is often a major issue, particularly when component models are developed independently for different purposes. This problem arises, for example, when one attempts to create a model that combines crop and hydrology models, crop and economic models, or crop and climate models . There have also been efforts, starting in the early 2000s, in which dynamic models have been developed to provide forecasts over aggregated areas . Traditionally, climate model output for a grid cell is down scaled to produce weather data time series for points that are then fed into crop models. However, the land surface also influences climate; processes within the atmosphere and oceans, and on the land, are coupled and dynamically interact over space on timescales from fractions of seconds to thousands of years. Crops are a major component of the land surface of the globe, occupying about a quarter of all land area. Regional climate can be sensitive to large-scale changes in cropped areas that can result from changes in economic or climate conditions . Therefore, another direction for agricultural impact assessments at a large-scale is to dynamically couple crop simulation with models of land and atmospheric processes.Osborne et al., 2009 showed that, in some parts of the world, the impact of changes in cropped area on regional surface temperature can be of the same magnitude as regional human-induced climate change.

This result raises the question of whether or not new fully-coupled climate change impacts studies will revise our previous estimates of food security impacts. It is clear that the full coupling of crop simulations within global climate models is opening up new possibilities for studies of the impact of climate change on agricultural production – studies that capture some of the complex and important feed backs within the Earth system at a large scale. Limitations in the skill of large-area modeling of crop production and yield is dominated by the density of data used in the simulation. More data should equate to better skill. However, the skill of large-scale modeling is determined by the smallest data set, whether this is the grid cell with the shortest run of observed yields, or the data grid with the largest resolution . We have seen recent increases in the resolution of climate input data and global grids of crop management and soil information. In this field of agricultural modeling, any future increase in data resolution should produce more skillful model simulations.We next discuss the state of current agricultural system science relative to its capabilities and limitations in providing information to assist a wide range of decision makers represented by the five Use Cases. Each Use Case contains a set of interactions between systems and users in a particular environment in a systems analysis. The Use Cases are for developing and developed country settings, demonstrating a range of needs for widely different applications at different scales and levels of intensification. Antle et al. indicated that these Use Cases need crop, livestock, and farming system models. The question that we address here is whether current agricultural system models, existing sources of data, and existing decision support systems are adequate for providing information needed for these Use Cases.Can existing crop, livestock, and farming system models, data, and ICT tools provide the information that Sizani needs to advise the small farmer? The short answer is “No”; there are currently no easily accessible and usable applications that would allow her to analyze the particular farmer’s situation. Or apps that can connect with models in the “cloud” to make runs needed for her to advise the farmer. Although there are models that partially meet her needs, and there are well documented examples of using models to develop insights on productivity enhancement strategies in the face of resource constraints and climate risk they have not been integrated or are not packaged for use by this type of non-expert user.

Models can, for example, simulate responses of crops to soil and weather conditions as well as water and nitrogen fertilizer input but do not generally simulate actual yield in production situations where, weeds, pests or diseases are not controlled. Two of the most serious limitations of many crop-soil models are their inabilities to accurately simulate soil infertility and their failure to represent losses associated with the wide range of pest, disease, and weed species that damage crops. In many intensive production systems, soil fertility, weeds, pests, and diseases are controlled so that responses in those situations can be represented by the costs of management inputs and the production responses to climate and water management. Typically, cropping system models simulate yields that are higher than actual yields in farmers’ fields, which are reduced due to poor management. In addition, fields are usually not homogeneous; for example, spacing between plants may vary considerably,whereas the models assume homogeneity. However, if pest and disease data are observed and available, these data can be input to some existing crop models to compute yield loss associated with specific pests and to diagnose the reasons for the gap between potential and actual yield . Keating and McCown have shown, however, that expert application of well adapted models can still lead to useful insights on many of the key constraints to productivity enhancement in small-holder situations. Generally, farming system models now in use have some capabilities needed to analyze this Use Case. However, most farming system models are not developed to be easily implemented by non-expert users nor for farms with characteristics different from those for which they were developed. An exception to this is the TOA-MD farming system model , although that model also needs reliable data from farm surveys to simulate a population of farms in contrast to a particular farm.It is impractical for Sizani to collect information on a particular farm, go back to her office and work with an analyst to evaluate options for the farmer. Instead, data are needed to describe a range of farming systems so that she could select the combination of biophysical, farming system, and household characteristics from available data. This would include information to allow her to tailor inputs to most closely match the conditions of specific farms. This includes climate, soil, management practice, labor and other inputs available for production and marketing of outputs, typical pest and disease pressures, availability and prices for farming inputs, and other farm, economic, and environmental information. Generally, sufficient data on the biophysical, environmental, and socio-economic conditions of each farm or for a range of farm typologies in the regions are not available. Although some data, such as climate and soil data, are available, generally these are not organized nor are they sufficiently site-specific that agricultural systems models can readily access them for analysis of specific farms. Although research has shown that some analyses needed to advise a farmer can be made, the availability of input data for agricultural systems models remains a major limitation.Most existing DSS tools that are available in Apps are focused on relatively narrow issues ,vertical grow rack such as when to apply a fungicide to a particular crop, when to apply the next irrigation, or how much N fertilizer to apply to a particular crop that will be grown on a particular type of soil in a specific setting. There are few DSS tools that make use of more integrated models to help advisors advise farmers in making farming system decisions . We envision a DSS platform that will connect various models, databases, analysis, and information synthesis tools in an easy-to-use interface for Sizani to set up the analyses and outputs to answer questions about the management of that particular farms’ biophysical and socioeconomic situation and the uncertainties in those estimates. Such DSS platforms are possible, but not yet constructed.Models of maize and other crops, livestock, and the farm household are also needed for this Use Case. These models are available for at least partially performing this type of analysis. Starting in the 1980s, several groups began using crop simulation models to evaluate alternative management systems in developing countries .

Models used in those efforts were generally based on CERES and other crop models now in DSSAT and on the ORYZA rice model developed by IRRI. More recently, the Global Futures and Harvest Choice CGIAR research projects led by the International Food Policy Research Institute have used crop and economic models to evaluate the potential benefits of developing new technologies, including new crop varieties . For example, Singh et al. used the DSSAT CROPGRO groundnut model with climate and soil inputs at six locations in India to evaluate different crop traits being targeted by CGIAR plant breeders. They found that the effect of combining various traits was beneficial, with estimated yield gains varying, depending on location and climate change conditions. Rapid advances in biotechnology and molecular plant breeding are helping researchers incorporate molecular markers and genes into models so that ultimately genetic composition of crops can be used to predict performance of future varieties to help target expensive and time consuming plant breeding efforts . The paper by Hwang et al. presents some concepts now being explored for next generation crop models. Similarly, considerable work has been done on farming system models to evaluate options for improving the livelihoods of farmers. These include farm simulation models , optimization models that attempt to select the best combination of enterprises and their management to achieve one or multiple goals of the farmer . Also, the Trade off Analysis model is currently being used as the basis for model-based impact assessments . Furthermore, this approach can incorporate results from crop and livestock models, as well as environmental and social outcome models, and it can be adapted for smallholder or large commercial farming systems. However, there are important limitations in the capabilities of these models, similar to those mentions in Use Case 1 Thus, there may be large yield gaps between actual yields in farmers’ fields and the potential productivity in those fields . When water, nitrogen, and climate are the major limitations in crop productivity, current models are highly useful, assuming that soil, weather, cultivar, and management input data are available for the analyses. In this Use Case, it is likely that other factors, including other soil nutrients, pests, diseases, and weeds, need to be taken into account. The challenge for next generation models includes not only modeling those factors but also collecting data that describe the production situation with all of the important yield-limiting and reducing factors. Another question is whether existing biophysical models can predict performance of the wide range of intensification options that may be used by farmers for this Use Case.

Digital agriculture’s strategy of overcoming hunger by increasing yield thereby may even exacerbate it

A variety of labels have been used for this emergent industry: precision agriculture, e-agriculture, smart agriculture, and digital agriculture, among others. Despite early critical use of precision agriculture, the term tends to be used in the industry to signify a specific suite of production-oriented technologies.However, information technologies are also used to open new markets and new territories for production. For example, digital platforms have become increasingly important for individual producers to bring their goods to market. Figure 1 shows how information technologies are intertwined throughout the cycle of agricultural production and sale.We use digital agriculture for its semantic breadth and increasing currency. In our taxonomy, precision agriculture is a subset of digital tools which improve efficiency through careful management of inputs. Three other types of tools—marketplace and financial platforms, e-extension, and smallholder management—are typically platform-based systems that mediate the social relation between farmers and the outside world. Marketplace and financial technologies help farmers access new credit lines and optimize their market behavior. E-extension is the digitalization of the practice of implementing technological innovations through farmer education, particularly in the international development context. E-extension, like the analog version that preceded it, dutch buckets system is largely reliant on insights produced far from the farm.

Finally, smallholder management platforms allow larger agribusinesses to exert control over smallholder farmers through close management of their inputs, products, and so forth. This may allow major actors to divest themselves of the risk inherent in owning land and instead subcontract smallholders in a relationship analogous to other platforms in the gig economy.For digital agriculture’s boosters, it has the potential to be the much-needed “fourth agricultural revolution” . In particular, it is framed as a climate-friendly way to feed the world and improve the lot of farmers around the world. By making the application of inputs more efficient, digital agriculture can indeed lessen the environmental impact and yield of agriculture. By increasing input efficiency and improving knowledge of market demand, digital agriculture may indeed improve the fortunes of producers. The rhetoric is not dishonest, but it is incomplete.Optimizing inputs enables the continued use of ecologically-harmful chemicals and practices, which would otherwise be abandoned if their effects were not actively mitigated . Digital agriculture’s marketing claims it will improve efficiency, increasing yield and minimizing the use of inputs—many of which are harmful and unsustainable. The externalities produced by using these inputs are the “un- and undervalued costs of industrial capitalist agriculture” .

A team at Cornell, for example, has developed a model that recommends ideal fertilizer application rates for each section of a farmer’s field in order to minimize nitrogen run of into the Gulf of Mexico, which causes algal blooms, depletes oxygen levels in the water, and kills fish and wildlife.While optimization limits the short-term damage of unsustainable practices, it also makes those practices more politically permissible and financially feasible. Thus, by making unsustainable practices appear sustainable, the necessity of adopting more ecologically and socially sustainable and just practices is delayed. By focusing on input management, these technologies advance a limited interpretation of sustainability that still depends on of-farm inputs, rather than a more radical shift to permanently sustainable practices . Just as digital agriculture promises to minimize inputs, it also promises to maximize yield—yet yield is not the problem. In the 1970s Amartya Sen noted that while starvation was increasing globally, food per capital was also increasing —as population grew, food production grew at a greater rate, not only globally but even regionally. While some scholars have taken issue with Sen’s empirical basis, an updated analysis using 2010 statistics found the same results . The direct relationship between hunger and food per capital, when we would expect an inverted one, betrays the simple thesis that hunger is due to a lack of food availability. Instead, Sen attributes hunger to an inability to exchange for food. Davis similarly notes the disconnect between food availability and hunger, finding that famine can occur in areas of grain surplus because it is more attributable to rural food management and exploitation than to production .

The “solution” to hunger, then, lies not in yield. Yield has increased; food per capital has increased; hunger persists. Therefore, stretching yield through digital agriculture is insufficient and does not address the political-economic basis of systemic hunger.The third key claim made by digital agriculture’s boosters is that it will improve farmers’ welfare, in particular their profits. Profits may be found in better decision-making, better yields, and better access to market information . In the Global North, such increased profits may be plausible. However, a primary mode for digital agriculture, the platform service, means that the data produced typically becomes the property of the platform provider. Weersink et al. note that a key challenge for digital agriculture is making this data useful; this, in turn, may favor larger companies with the capacity to process the data. Bronson notes this dynamic and warns that it may reproduce the distributional effects of the Green Revolution—that is, to concentrate wealth and power in the hands of major agribusinesses. In the Global South, digital agriculture presents a different set of problems for farmers’ welfare. Technological innovation that increases a crop’s yield in turn increases supply and undercuts the socially necessary labor time required to produce it. This dynamic lowers the crop’s exchange value at the expense of those at the bottom of global commodity chains, in particular the growers’ compensation per unit of crop. As this price drop is not accompanied by any increase in production for farmers without access to this technological innovation, this drop translates to lower overall compensation and to “exchange entitlement decline” . If they depend on exchange for subsistence, the decreased compensation translates to hunger as well.In reflecting on these mainstream claims, a different theme emerges. Rather than sustainability, nourishment, or farmer welfare, digital agriculture is fundamentally about securing the conditions to generate profit in the food system. Crucially, however, this is not about profit in food production alone, but in the wider capitalist economy for which food is obviously a fundamental input. Therefore, we submit that digital agriculture must be understood as addressing a specific set of crisis tendencies that have emerged at a particular juncture in the social, ecological, and spatial history of capitalism. This juncture is defined by interlocking moments of ecological disaster; enormous advances in information production, gathering, and processing; and “hypertrophic” urbanization . In this section we argue that rather than a solution to the climate crisis, hunger, or farmer welfare, the rise of digital agriculture can better be understood as an attempt to overcome crisis tendencies of “the relentless growth imperatives of an accelerating, increasingly planetary formation of capitalist urbanization” .

After briefly excavating the informational dynamics latent within the framework of extended and concentrated urbanization, we describe how digital agriculture functions as a “data fix” by allowing the intensification of agricultural industrialization and the extraction and enclosure, for eventual profit, of the data produced by digital agriculture technologies. An early theme in globalization literature was a tendency to embrace the rise of information technologies in a way that dematerialized the now planetary systems of extraction, production, and consumption . Such concepts, however, have largely been absorbed by analyses which show that a deterritorialized “information society” is not displacing traditional modes of production and social relations as much as emerging as a financial-managerial stratum in a “new international division of labor.” Another major theme in globalization studies is the ‘global city network,’ a set of nodes in the global space of flows from which the global economy could be commanded and controlled . In describing such cities as “strategic sites where global processes materialize” , they appear to be material sites floating in a sea of immaterial processes. In this model, cities are simultaneously the result of, yet alienated from, specific material processes— such as agricultural production—taking place beyond their bounds. In both concepts the informational nature of globalization is over-emphasized at the expense of its material effects. In an era of climate crisis, this shortcoming is glaring.One response has been to radically reframe globalization as a material process of urbanization,dutch buckets which unfolds as the product of dialectically-entwined moments of extension and concentration . Concentrated urbanization signifies the moment of agglomeration where the material flows of global capitalism accumulate into cities, megalopolises, and mega-regions. On the flip side, extended urbanization is the moment where remote territories are enclosed and transformed into operational landscapes that funnel energy, materials, and food into areas of accumulation. Both moments cause and are caused by the other: “The urban unfolds into the countryside just as the countryside folds back into the city” . Global capitalist urbanization is a metabolic process of moving and consuming the material world . This involves both fragmentation and homogenization —for example, the simultaneous expansion of monoculture agriculture and of liberal private property regimes. At the same time, enclosure and technological advances deprive peasants of their livelihoods; ‘depeasantization’ is the mirror of urbanization. However, the desire to develop a more materialist model of globalization leads to the black-boxing of information‘s role in facilitating vast networks of production and exchange. To bring information back in requires recognizing that something happens at the moment of concentration which sets the stage for extension. In the present framework, production and the growth imperative drive a search for more raw materials. But extension also depends on informational infrastructure to make a massively decentralized network of global supply chains profitable. Indeed, another way to describe capitalist geography is as “a skein of somewhat longer networks that rather inadequately embrace the world on the basis of points that become centers of calculation” . Information, along with material, is being drawn inwards in the moment of concentration; the processing of raw information—which is “what remains after one abstracts from the material aspects of physical reality” —into actionable knowledge informs extension processes. “Information processing” is computation, and computation at the scale required to make legible the vast amounts of data produced in the contemporary economy involves enormous physical infrastructural investment in data centers, undersea cables, and satellite networks . Such computational capital consists also of intellectual and human capital in the form of models, algorithms, and the expertise to deploy them.

There is a potential for the over accumulation of computational capital, however; as a result, there is a constant drive for firms to find productive outlets. This is what leads firms like Amazon, Microsoft, Google, Oracle, and Cisco—as well as funds invested in and consultancies hired by them—into digital agriculture. By locating, extracting, and enclosing data relevant to another materially productive sector , a firm like Amazon—whose cloud computing infrastructure Jef Bezos has compared to power utilities—can continue to grow. This applies at the worker level, too. Just as a glut of NASA-trained engineers and physicists became quants for hedge funds after the Space Race , a glut of software engineers and data scientists which Silicon Valley cannot absorb find employment outside of the tech sector, including at digital agriculture startups or divisions within larger agribusinesses. Indeed, agribusiness are planning for a future in which they become tech companies themselves: the head of digital agriculture at Bayer Monsanto, for example, has described the future of the conglomerate as a digital platform . The fundamental material crisis that digital agriculture attempts to fix through the manipulation of data is in the sociol-metabolic processes of capitalism and capitalist urbanization. To support social reproduction for a growing non-agrarian population, present-day industrial agriculture destroys its own ecological foundations. As the consequences of climate change become ever more apparent and render growing conditions ever more difficult, a new ecological regime is needed to prolong the production of cheap food and ensure future accumulation in the face of known crises . But not only is fossil fuel-based industrial agricultural production partially responsible for climate change—up to one-fifth of all greenhouse gas emissions—it also exhausts the ecologies within which it is practiced. The search for the fourth agricultural revolution is not a straightforward matter of addressing a Malthusian crisis of natural population growth, but a crisis of capitalism itself. This crisis tendency arises from capitalism’s dependency on the “four cheaps”— labor, food, energy, raw materials—to maintain each cycle of accumulation.

We refer to this strategy as the Agriculture for Development sequence

During the rest of the year, there are much less employment opportunities for rural than urban households, with the former working about half the time worked by urban households during the low season . Lack of labor smoothing across months can thus be a major cause of income differentials between rural and urban households. Measuring annual labor productivity as median household real consumption per capita, rural households are at 57% of individuals in urban households. When this is measured not per year but per hour worked, rural households are at 81% of individuals in urban households. With high urban unemployment in Malawi limiting the option of reducing rural poverty through permanent or seasonal rural-urban migration, this suggests that a key instrument for rural poverty reduction is to have less idle time for land and labor throughout the monthly calendar. For Bangladesh, Lagakos et al. proposed filling labor calendars for rural households through migration to cities during the lean season. When this option is not available due to high urban unemployment filling and smoothing labor calendars in rural areas becomes a key dimension of poverty reduction. This can involve employment both in agriculture with more diversified farming systems and in the local rural non-farm economy. This is the purpose of the agricultural and rural transformations that are important in redefining how to use agriculture for development.

Based on work done for the IFAD Rural Development Report led by Binswanger, for China by Huang , by BRAC on graduating the ultra-poor out of poverty ,nft hydroponic for the Gates Foundation by Boettiger et al. , and for the ATAI project , a strategy of using agriculture for development would involve the following five steps: Asset building, Green Revolution, Agricultural Transformation, Rural Transformation, and ultimately Structural Transformation as described in Table 1.Minimum asset endowments for SHF under the form of land, capital, health, knowledge and skills, and social capital are needed to initiate production for the market and participation in a value chain. This corresponds to minimum capital endowments to get started in production in farm household models such as Eswaran and Kotwal’s , and to asset thresholds to escape poverty traps in Barrett and Carter . The BRAC graduation model for the rural ultra-poor thus importantly starts with achieving minimum asset thresholds for households to engage in self-employment in agriculture , with rigorous impact evaluations demonstrating success in raising household consumption in five of six case countries. Evaluation with a randomized experiment of a BRAC credit program for landless workers and SHF in Bangladesh shows that loans can be used to achieve minimum asset endowments by renting land and selecting more favorable fixed rent over sharecropping contracts . The Green Revolution, whereby productivity growth is achieved in staple crops through the adoption and diffusion of high yielding variety seeds and fertilizers is the initial step in agricultural modernization. It has been actively pursued to achieve food security and is a learning ground for the subsequent transformations of agriculture and rural areas.

It has been a major success of the Consultative Group in International Agricultural Research and is still an ongoing effort in Sub-Saharan Africa and Eastern India. A key objective of the Agricultural Transformation is to fill in rural households’ labor calendars over as much of the year as possible through multiple cropping — which typically requires water control to cultivate land in the dry season–, the development of value chains for new crops, and contracting among agents in these value chains. An example is the introduction of short duration rice varieties in Bangladesh that frees the land for an additional crop, typically high value products such as potatoes and onions, between rainy season and dry season rice crops. This makes an important contribution to filling land and labor calendars and to reducing the length of the hungry season . Because the Agricultural Transformation implies diversification of farming systems, it is a key element of national food security strategies where diverse diets, including perishable goods such as fruits and vegetables, dairy products, and meats that are less traded than staple foods, are an important element of healthy diets . SHFs are engaged in value chains that define the way they relate to markets. Value chains for agricultural products link farmers backward to their input and technology suppliers and forward to intermediaries, processors, and ultimately consumers . Relations within value chains often take the form of contractual arrangements. Induced by income gains for consumers, urbanization, and globalization, there has been in recent years a rapid development of value chains not only for low-value staple food crops, but also for medium value traditional domestic consumption and export crops, and high-value non-traditional export crops. Their structure can take a wide variety of forms in linking SHF to consumers, ranging from traditional spot markets to elaborate contract farming, productive alliances , and out-grower schemes.Contracts can be “resource-providing”, thus contributing to solve market and institutional failures for participating SHFs.

A key objective of the Rural Transformation is to give access to smallholder households to sources of income beyond agriculture. In Ghana, income derived from the rural non-farm economy for rural households is about 40% of total income, a share that increases as land endowments fall . It is indeed the case that, with land limitations, smallholder households rarely exit poverty with agriculture alone. A rural transformation requires the development of land markets and of labor markets . This process will typically happen first in the more favorable areas where a rural non-farm economy linked to agriculture can develop through forward, backward, and final demand linkages. It corresponds to the Agriculture Demand-Led Industrialization strategy advocated by Adelman and Mellor that is actively pursued in countries such as Ethiopia and Rwanda, and through CAADP in much of Sub-Saharan Africa. There are basically two contrasted approaches to potentially overcoming the problems that obstruct an Agriculture for Development sequence. The first consists in focusing on particular groups of farmers and addressing each of the problems in their own shapes and forms that affect them in modernizing. We can label this a “supply-side” approach to modernization and transformations. It consists in securing the existence and profitability of innovations, ensuring their local availability, and then overcoming each of the four major constraints to demand and adoption through either better technology or through institutional innovations . The agents for this approach are principally public and social such as governments, development agencies, NGOs, and donors. The second consists in creating incentives for SHF to modernize by building value chains for the particular product, and managing vertical and horizontal coordination within the value chains to overcome the profitability-availability-constraints obstacles as they apply to inclusion and competitiveness of SHF in the value chain. This is a “demand-side” approach to modernization and transformations. It consists in creating the demand for innovations in order to establish SHF competitiveness within a value chain, and then securing the existence, availability, and conditions for adoption of innovations. The approach thus requires both value chain development and value chain inclusion of SHFs. In this case, the agents are principally private such as enterprises and producer organizations for contracting, and lead firms, multi-stakeholder platforms, and benevolent agents for coordination. Public-private partnerships can be found among both supply- and demand-side initiatives.Technological innovation are first analyzed in experimental plots, usually for yield and resilience to specific shocks. But this does not tell us whether the innovation is likely to be adopted by SHF. Analysis of the adoption problem should start with verification that the innovation is indeed profitable for the intended SHF under their own circumstances, objectives, and capacities. Measuring profitability in farmers’ plots is however very difficult . There are data problems in observing family labor time and definitional problems in establishing the opportunity cost for family labor and self-provided inputs. Conditions also vary year-to-year due to weather conditions, with only short time series to observe how climate affects outcomes, made even more difficult to interpret with climate change. And there are many unobservable conditions and complementary factors that affect profitability and compromise the external validity of any measurement made at a particular time and place. An alternative approach is to verify profitability without measuring it. Some among the best endowed and best located farmers have to be able to make sustained use of the innovation for the innovation to have adoption potential by others under current market, policy, nft system and complementary input conditions. This can be established by observation, experimentation, or simulation.

Once the innovation is proven profitable and is locally available, its adoption may still be hampered by constraints facing SHF in accessing liquidity, risk-reducing instruments, information, and markets. These four categories of constraints have been extensively analyzed using in particular randomized control trials to identify their causal relations to adoption . These studies typically seek to identify ways of overcoming these constraints that could be implemented by governments, international organizations, NGOs, and benevolent agents such as philanthropic foundations and corporate social responsibility initiatives. Due to seasonality, especially under rainfed farming conditions which is where most of the lag in modernization currently prevails , there is a lack of correspondence between the timing of agricultural incomes and that of expenditures. As a consequence, the inter-temporal displacement of liquidity through credit and savings appears to be important for farmers to invest in new technologies, purchase inputs, optimize the timing of sales, buy consumption goods, and cover timely expenditures such as school fees. Financial services for SHFs appear to frequently be ill-designed for their purpose, expensive, excessively risky, and not easily available. Even when they have formal land titles, SHF are typically unwilling to put their land at risk as collateral with a commercial bank, thus acting as “risk constrained” . Microfinance products that effectively circumvent the collateral problem by relying on group lending and joint liability tend to be too expensive for the long agricultural cycles and have repayment conditions that are typically ill adapted to the timing of farmers’ capacity to pay . Availability of credit from formal sources, both commercial and non-profit, is consequently limited, and SHFs must either self-finance or rely on informal lenders with prohibitive interest rates. Hence, there would appear to exist a largely unresolved liquidity constraint on adoption originating on the supply side of the financial market. Yet, this is often not the main reason for low adoption which may be on the demand side. Recent field experiments are providing evaluations of interventions aiming at relaxing the liquidity constraint on SHFs, with fertilizer the most commonly used indicator of technology adoption because of its ubiquitous recognition and yet massive under use. While contexts and interventions vary for these experiments, they surprisingly tend to show that a liquidity constraint is not the reason why a majority of SHFs are under-investing in fertilizers. The main constraint may be instead lack of profitability in adopting fertilizers.A first category of experiments consists in providing unrestricted access to credit to a defined eligible population, as was done in Morocco , Mali , and Ethiopia . While interest rates in these studies were variously subsidized , uptake remained low: only 17% of eligible farmers took a loan in Morocco, 21% in Mali, and 36% in Ethiopia. Furthermore, farmers that did take a loan only used a small fraction of the liquidity to increase their expenditures on fertilizer or other agricultural inputs . Other experiments offered restricted credit that can only be used to purchase agricultural inputs. Such credit displaces the equilibrium allocation of liquidity in favor of the targeted inputs, similarly to what a price discount would do. And yet, uptake remained low. In Malawi input credit for high-yielding maize and groundnuts was taken by 33% of the farmers . This low demand for credit thus seems to be reflective of a low demand for the inputs themselves. Low demand for fertilizer is exemplified in two rather extreme experiments. In Mali, Beaman et al. provided to another group of farmers a pure cash grant, rather than the credit described above. This only increased expenditures on fertilizer by 15%, in comparison with 11% with a credit that had to be paid for, showing that credit is not the major constraint to adoption.