All the supernatant was discarded and the pellet was dried briefly in a vacuum desiccator

To generate the sog1-7 and suv2-3 mutations, als3-1 seeds were treated with EMS. M2 seedlings were screened for mutants that suppressed the hypersensitivity of als3-1 to Al in a 0.75 mM AlCl3 gel soaked environment. EMS treated seeds were planted around the periphery of the plates and were grown for 7 days at 20°C with …

Case mothers moved more in the latter half of the first year but this could be due to random variation

For cases and all controls, we examined the agreement between exposures during the first year of life assigned to birth residence and those assigned to LexisNexis addresses. Restricting to cases diagnosed at or after 1 year of age, we assessed the level of agreement between exposures assigned to birth residence and those assigned to diagnosis …